Single yeast cell nanomotions correlate with cellular activity.

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State: Public
Version: Final published version
License: CC BY-NC 4.0
Serval ID
serval:BIB_3C6EA13A3ECB
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Single yeast cell nanomotions correlate with cellular activity.
Journal
Science advances
Author(s)
Willaert RG, Vanden Boer P., Malovichko A., Alioscha-Perez M., Radotić K., Bartolić D., Kalauzi A., Villalba MI, Sanglard D., Dietler G., Sahli H., Kasas S.
ISSN
2375-2548 (Electronic)
ISSN-L
2375-2548
Publication state
Published
Issued date
06/2020
Peer-reviewed
Oui
Volume
6
Number
26
Pages
eaba3139
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Living single yeast cells show a specific cellular motion at the nanometer scale with a magnitude that is proportional to the cellular activity of the cell. We characterized this cellular nanomotion pattern of nonattached single yeast cells using classical optical microscopy. The distribution of the cellular displacements over a short time period is distinct from random motion. The range and shape of such nanomotion displacement distributions change substantially according to the metabolic state of the cell. The analysis of the nanomotion frequency pattern demonstrated that single living yeast cells oscillate at relatively low frequencies of around 2 hertz. The simplicity of the technique should open the way to numerous applications among which antifungal susceptibility tests seem the most straightforward.
Pubmed
Web of science
Open Access
Yes
Create date
09/07/2020 12:16
Last modification date
04/02/2021 6:25
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