MAGE-A3 and MAGE-A4 specific CD4(+) T cells in head and neck cancer patients: detection of naturally acquired responses and identification of new epitopes.

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Ressource 1Download: serval:BIB_3C2CBE6BF26D.P001 (747.35 [Ko])
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Serval ID
serval:BIB_3C2CBE6BF26D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
MAGE-A3 and MAGE-A4 specific CD4(+) T cells in head and neck cancer patients: detection of naturally acquired responses and identification of new epitopes.
Journal
Cancer Immunology, Immunotherapy
Author(s)
Cesson V., Rivals J.P., Escher A., Piotet E., Thielemans K., Posevitz V., Dojcinovic D., Monnier P., Speiser D., Bron L., Romero P.
ISSN
1432-0851[electronic], 0340-7004[linking]
Publication state
Published
Issued date
2011
Volume
60
Number
1
Pages
23-35
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Frequent expression of cancer testis antigens (CTA) has been consistently observed in head and neck squamous cell carcinomas (HNSCC). For instance, in 52 HNSCC patients, MAGE-A3 and -A4 CTA were expressed in over 75% of tumors, regardless of the sites of primary tumors such as oral cavity or hypopharynx. Yet, T-cell responses against these CTA in tumor-bearing patients have not been investigated in detail. In this study, we assessed the naturally acquired T-cell response against MAGE-A3 and -A4 in nonvaccinated HNSCC patients. Autologous antigen-presenting cells pulsed with overlapping peptide pools were used to detect and isolate MAGE-A3 and MAGE-A4 specific CD4(+) T cells from healthy donors and seven head and neck cancer patients. CD4(+) T-cell clones were characterized by cytokine secretion. We could detect and isolate MAGE-A3 and MAGE-A4 specific CD4(+) T cells from 7/7 cancer patients analyzed. Moreover, we identified six previously described and three new epitopes for MAGE-A3. Among them, the MAGE-A3(111-125) and MAGE-A3(161-175) epitopes were shown to be naturally processed and presented by DC in association with HLA-DP and DR, respectively. All of the detected MAGE-A4 responses were specific for new helper epitopes. These data suggest that naturally acquired CD4(+) T-cell responses against CT antigens often occur in vivo in HNSCC cancer patients and provide a rationale for the development of active immunotherapeutic approaches in this type of tumor.
Pubmed
Web of science
Open Access
Yes
Create date
03/02/2011 14:29
Last modification date
01/10/2019 6:17
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