Targeted ultramicrotomy: a valuable tool for correlated light and electron microscopy of small model organisms.

Details

Serval ID
serval:BIB_3C184DD70E7E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Targeted ultramicrotomy: a valuable tool for correlated light and electron microscopy of small model organisms.
Journal
Methods in cell biology
Author(s)
Kolotuev I., Bumbarger D.J., Labouesse M., Schwab Y.
ISSN
0091-679X (Print)
ISSN-L
0091-679X
Publication state
Published
Issued date
2012
Peer-reviewed
Oui
Volume
111
Pages
203-222
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Correlative light and electron microscopy (CLEM) is used when one needs to combine both imaging modalities on the same sample. When working on living small model organisms, such as Caenorhabditis elegans, specific CLEM protocols are required to acquire high-resolution light microscopic images of a region of interest and thereafter to relocate and study the same object at the ultrastructural level using a transmission electron microscope. In this chapter, we describe how to process living specimens from the confocal microscope to the transmission electron microscopy (TEM), focusing on an improved ultramicrotomy technique that allows a precise and reliable targeting of the object of interest. This improvement significantly reduces the time consuming and frequently frustrating search for the region of interest. Our targeted ultramicrotomy protocol is versatile enough to be applied on a variety of bulk specimens, such as fly and fish embryos, or mouse tissues.
Keywords
Animals, Caenorhabditis elegans/ultrastructure, Cryoelectron Microscopy, Cryopreservation, Cryoultramicrotomy, Microscopy, Confocal, Microscopy, Electron, Transmission, Plastic Embedding, Sepharose/chemistry
Pubmed
Web of science
Create date
01/10/2021 9:39
Last modification date
29/07/2022 6:39
Usage data