Regulation of the intracellular distribution, cell surface expression, and protein levels of AMPA receptor GluR2 subunits by the monocarboxylate transporter MCT2 in neuronal cells.

Details

Serval ID
serval:BIB_3BDC99792448
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Regulation of the intracellular distribution, cell surface expression, and protein levels of AMPA receptor GluR2 subunits by the monocarboxylate transporter MCT2 in neuronal cells.
Journal
Journal of Neurochemistry
Author(s)
Maekawa F., Tsuboi T., Fukuda M., Pellerin L.
ISSN
1471-4159[electronic], 0022-3042[linking]
Publication state
Published
Issued date
2009
Peer-reviewed
Oui
Volume
109
Number
6
Pages
1767-1778
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The neuronal monocarboxylate transporter, MCT2, is not only an energy substrate carrier but it is also purported to be a binding partner for the alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor GluR2 subunit. To unravel a putative role of MCT2 in the regulation of GluR2 subcellular distribution, Neuro2A cells and primary cultures of mouse cortical neurons were co-transfected with plasmids containing sequences to express the fluorescent proteins mStrawberry (mStb)-fused MCT2 and Venus-fused GluR2. Subsequently, their subcellular distribution was visualized by fluorescence microscopy. GluR2 was led to form perinuclear and dendritic clusters together with MCT2 when co-transfected in Neuro2A cells or in neurons, following the original distribution of MCT2. MCT2 co-transfection had no effect on the intracellular distribution of several other post-synaptic proteins, although it partially affected the intracellular distribution of GluR1 similarly to GluR2. Both cell surface and total protein expression levels of GluR2 were significantly reduced by co-expression with MCT2. Finally, partial perinuclear and dendritic co-localization between MCT2 and Rab8, a member of the small GTPase family involved in membrane trafficking of AMPA receptors, was also observed in co-transfected neurons. These results suggest that MCT2 could influence AMPA receptor trafficking within neurons by modulating GluR2 sorting between different subcellular compartments.
Keywords
Animals, Cells, Cultured, Cerebral Cortex/cytology, Composite Resins/metabolism, Extracellular Fluid/metabolism, Gene Expression Regulation/drug effects, Gene Expression Regulation/physiology, Luminescent Proteins/genetics, Monocarboxylic Acid Transporters/genetics, Neuroblastoma/pathology, Neurons/cytology, Neurons/drug effects, Protein Transport/drug effects, Protein Transport/physiology, RNA, Small Interfering/pharmacology, Receptors, AMPA/genetics, Receptors, AMPA/metabolism, Transfection/methods, Vesicular Transport Proteins/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
18/11/2009 16:37
Last modification date
20/08/2019 13:31
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