Tet-On Systems For Doxycycline-inducible Gene Expression.

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State: Public
Version: Final published version
Serval ID
serval:BIB_3BB90FF42FA1
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
Tet-On Systems For Doxycycline-inducible Gene Expression.
Journal
Current gene therapy
Author(s)
Das A.T., Tenenbaum L., Berkhout B.
ISSN
1875-5631 (Electronic)
ISSN-L
1566-5232
Publication state
Published
Issued date
2016
Peer-reviewed
Oui
Volume
16
Number
3
Pages
156-167
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish

Abstract
The tetracycline-controlled Tet-Off and Tet-On gene expression systems are used to regulate the activity of genes in eukaryotic cells in diverse settings, varying from basic biological research to biotechnology and gene therapy applications. These systems are based on regulatory elements that control the activity of the tetracycline-resistance operon in bacteria. The Tet-Off system allows silencing of gene expression by administration of tetracycline (Tc) or tetracycline-derivatives like doxycycline (dox), whereas the Tet-On system allows activation of gene expression by dox. Since the initial design and construction of the original Tet-system, these bacterium-derived systems have been significantly improved for their function in eukaryotic cells. We here review how a dox-controlled HIV-1 variant was designed and used to greatly improve the activity and dox-sensitivity of the rtTA transcriptional activator component of the Tet-On system. These optimized rtTA variants require less dox for activation, which will reduce side effects and allow gene control in tissues where a relatively low dox level can be reached, such as the brain.

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02/02/2017 16:36
Last modification date
20/08/2019 13:31
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