IS431mec-mediated integration of a bleomycin-resistance gene into the chromosome of a methicillin-resistant Staphylococcus aureus strain isolated in Japan.

Details

Serval ID
serval:BIB_3B9147B2630F
Type
Article: article from journal or magazin.
Collection
Publications
Title
IS431mec-mediated integration of a bleomycin-resistance gene into the chromosome of a methicillin-resistant Staphylococcus aureus strain isolated in Japan.
Journal
Applied microbiology and biotechnology
Author(s)
Sugiyama M., Yuasa K., Bhuiyan M.Z., Iwai Y., Masumi N., Ueda K.
ISSN
0175-7598 (Print)
ISSN-L
0175-7598
Publication state
Published
Issued date
08/1996
Peer-reviewed
Oui
Volume
46
Number
1
Pages
61-66
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
A methicillin-resistant Staphylococcus aureus (MRSA) strain B-26, isolated clinically in Hiroshima University Hospital, is resistant to bleomycin together with kanamycin. In the present study, we analysed the nucleotide sequence of the 5.1-kb HindIII fragment containing the bleomycin- and kanamycin-resistance genes, which were previously cloned [Bhuiyan et al. (1995) Appl Microbiol Biotechnol 43: 65-69] from the chromosomal DNA of MRSA B-26. The present study found that the DNA sequence contains the duplicated target sequence (GATTAGAT) consisting of 8 bp for transposase and the entire nucleotide sequence of the plasmid pUB110, together with the sequence of inverted repeats (16 bp), designated IR-r and IR-1 in IS431 mec. The 8-bp duplication sequence, produced by the transposable element, was first found by us. We proposed that bleomycin resistance in MRSA B-26 is attributed to the IS431 mec-mediated integration of pUB110 into the chromosome.

Keywords
Bacterial Proteins, Base Sequence/genetics, Bleomycin/pharmacology, Chromosomes, Bacterial/genetics, DNA Transposable Elements/genetics, Drug Resistance, Microbial/genetics, Drug Resistance, Multiple/genetics, Genes, Bacterial/genetics, Japan, Kanamycin Resistance/genetics, Methicillin/pharmacology, Methicillin Resistance/genetics, Molecular Sequence Data, Plasmids/genetics, Restriction Mapping, Sequence Analysis, DNA, Staphylococcus aureus/drug effects, Staphylococcus aureus/genetics, Transformation, Bacterial
Pubmed
Web of science
Create date
01/03/2018 15:42
Last modification date
20/08/2019 13:31
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