Structural analysis of TCR-ligand interactions studied on H-2Kd-restricted cloned CTL specific for a photoreactive peptide derivative.

Details

Serval ID
serval:BIB_3B4AE7192954
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Structural analysis of TCR-ligand interactions studied on H-2Kd-restricted cloned CTL specific for a photoreactive peptide derivative.
Journal
Immunity
Author(s)
Luescher I.F., Anjuère F., Peitsch M.C., Jongeneel C.V., Cerottini J.C., Romero P.
ISSN
1074-7613
Publication state
Published
Issued date
07/1995
Peer-reviewed
Oui
Volume
3
Number
1
Pages
51-63
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
To study the interaction of the TCR with its ligand, the complex of a MHC molecule and an antigenic peptide, we modified a TCR contact residue of a H-2Kd-restricted antigenic peptide with photoreactive 4-azidobenzoic acid. The photoreactive group was a critical component of the epitope recognized by CTL clones derived from mice immunized with such a peptide derivative. The majority of these clones expressed V beta 1-encoded beta chains that were paired with J alpha TA28-encoded alpha chains. For one of these TCR, the photoaffinity labeled sites were mapped on the alpha chain as a J alpha TA28-encoded tryptophan and on the beta chain as a residue of the C' strand of V beta 1. Molecular modeling of this TCR suggested the presence of a hydrophobic pocket that harbors this tryptophan as well as a tyrosine on the C' strand of V beta 1 between which the photoreactive side chain inserts. It is concluded that this avid binding principle may account for the preferential selection of V beta 1 and J alpha TA28-encoded TCR.
Keywords
Amino Acid Sequence, Animals, Azides, Base Sequence, Binding Sites, Clone Cells, Immunization, Ligands, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Models, Molecular, Molecular Sequence Data, Protein Structure, Secondary, Receptors, Antigen, T-Cell/chemistry, Receptors, Antigen, T-Cell/immunology, T-Lymphocytes, Cytotoxic/immunology
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 15:39
Last modification date
20/08/2019 13:31
Usage data