Association of prolonged survival in HLA-A2+ progressive multifocal leukoencephalopathy patients with a CTL response specific for a commonly recognized JC virus epitope.

Details

Serval ID
serval:BIB_3B1CC0BAB915
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Association of prolonged survival in HLA-A2+ progressive multifocal leukoencephalopathy patients with a CTL response specific for a commonly recognized JC virus epitope.
Journal
Journal of Immunology
Author(s)
Koralnik I.J., Du Pasquier R.A., Kuroda M.J., Schmitz J.E., Dang X., Zheng Y., Lifton M., Letvin N.L.
ISSN
0022-1767[print], 0022-1767[linking]
Publication state
Published
Issued date
2002
Peer-reviewed
Oui
Volume
168
Number
1
Pages
499-504
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Abstract
The role of JC virus (JCV)-specific CTL was explored in the immunopathogenesis of progressive multifocal leukoencephalopathy (PML). We identified a 9-aa epitope of the JCV capsid protein VP1, the VP1(p100) peptide ILMWEAVTL, which is recognized by CTL of HLA-A2+ HIV+/PML survivors. We then constructed an HLA-A*0201/VP1(p100) tetrameric complex that allowed us to assess by flow cytometry the PBMC of 13 PML patients and 11 control subjects for the presence of JCV-specific CTL. VP1(p100)-specific CTL were detected by tetramer binding in VP1(p100)-stimulated PBMC of five of seven (71%) PML survivors and zero of six PML progressors (p = 0.02). Two of three HIV+ patients with a leukoencephalopathy resembling PML, but with no virologic evidence of JCV infection, also had detectable VP1(p100)-specific CTL in their PBMC. PBMC of eight HIV+ patients with other neurologic diseases and healthy control subjects had no detectable JCV-specific CTL. These data suggest that the JCV-specific cellular immune response may be important in the containment of PML, and the tetramer-staining assay may provide a useful prognostic tool in the clinical management of these patients.
Keywords
Antigens, Viral/immunology, Capsid/immunology, Capsid Proteins, Cells, Cultured, Cytotoxicity Tests, Immunologic, Epitopes, T-Lymphocyte/immunology, Flow Cytometry, HLA-A2 Antigen/analysis, Humans, JC Virus/immunology, Kinetics, Leukoencephalopathy, Progressive Multifocal/diagnosis, Leukoencephalopathy, Progressive Multifocal/immunology, Survivors, T-Lymphocytes, Cytotoxic/immunology
Pubmed
Web of science
Create date
25/01/2008 14:56
Last modification date
20/08/2019 13:30
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