Stiffness tomography exploration of living and fixed macrophages.

Details

Serval ID
serval:BIB_3B14E4D048B8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Stiffness tomography exploration of living and fixed macrophages.
Journal
Journal of Molecular Recognition
Author(s)
Roduit C., Longo G., Benmessaoud I., Volterra A., Saha B., Dietler G., Kasas S.
ISSN
1099-1352 (Electronic)
ISSN-L
0952-3499
Publication state
Published
Issued date
2012
Peer-reviewed
Oui
Volume
25
Number
5
Pages
241-246
Language
english
Abstract
Stiffness tomography is a new atomic force microscopy imaging technique that allows highlighting structures located underneath the surface of the sample. In this imaging mode, such structures are identified by investigating their mechanical properties. We present here, for the first time, a description of the use of this technique to acquire detailed stiffness maps of fixed and living macrophages. Indeed, the mechanical properties of several macrophages were studied through stiffness tomography imaging, allowing some insight of the structures lying below the cell's surface. Through these investigations, we were able to evidence the presence and properties of stiff column-like features located underneath the cell membrane. To our knowledge, this is the first evidence of the presence, underneath the cell membrane, of such stiff features, which are in dimension and form compatible with phagosomes. Moreover, by exposing the cells to cytochalasin, we were able to study the induced modifications, obtaining an indication of the location and mechanical properties of the actin cytoskeleton. Copyright © 2012 John Wiley & Sons, Ltd.
Keywords
stiffness tomography, AFM, macrophage, mechanical properties, cytoskeleton
Pubmed
Web of science
Create date
23/05/2012 10:15
Last modification date
20/08/2019 13:30
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