Comparison between GC-MS and the EMIT II, Abbott ADx, and Roche OnLine immunoassays for the determination of THCCOOH.

Details

Serval ID
serval:BIB_3A714B0E6F65
Type
Article: article from journal or magazin.
Collection
Publications
Title
Comparison between GC-MS and the EMIT II, Abbott ADx, and Roche OnLine immunoassays for the determination of THCCOOH.
Journal
Journal of Analytical Toxicology
Author(s)
Kintz P., Machart D., Jamey C., Mangin P.
ISSN
0146-4760[print], 0146-4760[linking]
Publication state
Published
Issued date
1995
Volume
19
Number
5
Pages
304-306
Language
english
Abstract
Gas chromatography-mass spectrometry (GC-MS) and immunological methods, including the Syva enzyme multiplied immunoassay technique, the Abbott fluorescence polarization immunoassay, and the Roche OnLine immunoassay, were compared for the determination of 11-nor-delta 9-tetrahydrocannabinol-9- carboxylic acid (THCCOOH). The results of all three immunoassays were not in accordance with the GC-MS results in three cases of a 72-specimen panel. Only one false negative was observed using the OnLine immunoassay. The immunological methods compared favorably and are acceptable for detecting the presence of cannabis metabolites in urine. These results support the concept that all immunoassays for cannabinoids should be considered as screening procedures. No concentration correlation between GC-MS and the immunoassays could be established because of the different cross-reactivities of the metabolites.
Keywords
Calibration, Enzyme Multiplied Immunoassay Technique/standards, Fluorescence Polarization Immunoassay/standards, Gas Chromatography-Mass Spectrometry/standards, Humans, Reagent Kits, Diagnostic, Tetrahydrocannabinol/urine
Pubmed
Create date
19/10/2010 15:38
Last modification date
20/08/2019 13:30
Usage data