Differential expression of mitochondrial genes between queens and workers during caste determination in the honeybee Apis mellifera.

Details

Serval ID
serval:BIB_39C039ADEEE7
Type
Article: article from journal or magazin.
Collection
Publications
Title
Differential expression of mitochondrial genes between queens and workers during caste determination in the honeybee Apis mellifera.
Journal
The Journal of Experimental Biology
Author(s)
Corona M., Estrada E., Zurita M.
ISSN
0022-0949
Publication state
Published
Issued date
1999
Peer-reviewed
Oui
Volume
202
Number
Pt 8
Pages
929-938
Language
english
Abstract
The nourishment received by female honeybee larvae determines their differentiation into queens or workers. In this study, we report the first molecular analysis of differences that occur between queens and workers during the caste-determination process. RNA-differential display experiments identified a clone that encodes for a gene that is homologous to the nuclear-encoded mitochondrial translation initiation factor (AmIF-2mt). Semi-quantitative analysis by reverse transcriptase/polymerase chain reaction (RT-PCR) throughout honeybee development detected a higher level of expression of this gene in queen larvae than in worker larvae. Analysis of two other genes encoding mitochondrial proteins, cytochrome oxidase subunit 1 (COX-1; mitochondrial-encoded) and cytochrome c (cyt c; nuclear-encoded) also showed differential expression of these two genes between queens and workers. In particular, the cyt c transcript is more abundant in queen larvae and throughout the metamorphosis of the queen. These results indicate that the higher respiratory rate previously documented in queen larvae is accomplished through a higher level of expression of both nuclear- and mitochondrial-encoded genes for mitochondrial proteins.
Keywords
Amino Acid Sequence, Animals, Base Sequence, Bees/genetics, Bees/growth &amp, development, Cytochrome c Group/genetics, DNA Primers/genetics, DNA, Complementary/genetics, DNA, Mitochondrial/genetics, Electron Transport Complex IV/genetics, Female, Gene Expression Regulation, Developmental, Genes, Insect, Insect Proteins/genetics, Molecular Sequence Data, Peptide Initiation Factors/genetics, RNA, Messenger/genetics, RNA, Messenger/metabolism, Sequence Homology, Amino Acid
Pubmed
Web of science
Create date
10/03/2008 11:56
Last modification date
20/08/2019 13:29
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