Ionic liquids as matrices in microfluidic sample deposition for high-mass matrix- assisted laser desorption/ionization mass spectrometry.

Details

Serval ID
serval:BIB_38AA73DB96D2
Type
Article: article from journal or magazin.
Collection
Publications
Title
Ionic liquids as matrices in microfluidic sample deposition for high-mass matrix- assisted laser desorption/ionization mass spectrometry.
Journal
European journal of mass spectrometry
Author(s)
Weidmann S., Kemmerling S., Mädler S., Stahlberg H., Braun T., Zenobi R.
ISSN
1469-0667 (Print)
ISSN-L
1469-0667
Publication state
Published
Issued date
2012
Peer-reviewed
Oui
Volume
18
Number
3
Pages
279-286
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Sample preparation for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) via a microfluidic deposition device using ionic liquid matrices addresses several problems of standard protocols with crystalline matrices, such as the heterogeneity of sample spots due to the co-crystallization of sample and matrix and the limited capability for high-throughput analysis. Since ionic liquid matrices do not solidify during the measurement, the resulting sample spots are homogeneous. The use of these matrices is also beneficial for automated sample preparation, since crystallization of the matrix is avoided and, thus, no clogging of the spotting device can occur. The applicability of ionic liquids to the analysis of biomolecules with high molecular weights, up to ≈ 1 MDa is shown, as well as a good sensitivity (5 fmol) for recombinant human fibronectin, a protein with a molecular weight of 226 kDa. Microfluidic sample deposition of proteins with high molecular weights will, in the future, allow parallel sample preparation for MALDI-MS and for electron microscopy.
Keywords
Animals, Cattle, Crystallization, Fibronectins/analysis, Fibronectins/chemistry, Humans, Immunoglobulin G/analysis, Immunoglobulin G/chemistry, Immunoglobulin M/analysis, Immunoglobulin M/chemistry, Ionic Liquids/chemistry, Microfluidics/methods, Microfluidics/standards, Molecular Weight, Proteomics/methods, Proteomics/standards, Reproducibility of Results, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/standards, Thyroglobulin/analysis, Thyroglobulin/chemistry
Pubmed
Web of science
Create date
09/06/2023 16:03
Last modification date
28/07/2023 6:59
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