Biomedical nanoparticles modulate specific CD4(+) T cell stimulation by inhibition of antigen processing in dendritic cells

Details

Serval ID
serval:BIB_38A74A784201
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Biomedical nanoparticles modulate specific CD4(+) T cell stimulation by inhibition of antigen processing in dendritic cells
Journal
Nanotoxicology
Author(s)
Blank F., Gerber P., Rothen-Rutishauser B., Sakulkhu U., Salaklang J., De Peyer K., Gehr P., Nicod L.P., Hofmann H., Geiser T., Petri-Fink A., Von Garnier C.
ISSN
1743-5390
Publication state
Published
Issued date
2011
Peer-reviewed
Oui
Volume
5
Number
4
Pages
606-621
Language
english
Notes
Publication type : Article
Abstract
Understanding how nanoparticles may affect immune responses is an essential prerequisite to developing novel clinical applications. To investigate nanoparticle-dependent outcomes on immune responses, dendritic cells (DCs) were treated with model biomedical poly(vinylalcohol)-coated super-paramagnetic iron oxide nanoparticles (PVA-SPIONs). PVA-SPIONs uptake by human monocyte-derived DCs (MDDCs) was analyzed by flow cytometry (FACS) and advanced imaging techniques. Viability, activation, function, and stimulatory capacity of MDDCs were assessed by FACS and an in vitro CD4(+) T cell assay. PVA-SPION uptake was dose-dependent, decreased by lipopolysaccharide (LPS)-induced MDDC maturation at higher particle concentrations, and was inhibited by cytochalasin D pre-treatment. PVA-SPIONs did not alter surface marker expression (CD80, CD83, CD86, myeloid/plasmacytoid DC markers) or antigen-uptake, but decreased the capacity of MDDCs to process antigen, stimulate CD4(+) T cells, and induce cytokines. The decreased antigen processing and CD4(+) T cell stimulation capability of MDDCs following PVA-SPION treatment suggests that MDDCs may revert to a more functionally immature state following particle exposure.
Keywords
SPIONs, dendritic cells, immune response, antigen processing, antigen presentation, IRON-OXIDE NANOPARTICLES, IN-VITRO, RESPIRATORY-TRACT, OXIDATIVE STRESS, PARTICLE UPTAKE, ALLERGEN, SURFACE, VIVO, IMMUNOTHERAPY, ADJUVANTS
Web of science
Create date
08/12/2011 12:14
Last modification date
20/08/2019 13:28
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