Developmental life cycle of Leishmania--cultivation and characterization of cultured extracellular amastigotes

Details

Serval ID
serval:BIB_3861C9E05521
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
Developmental life cycle of Leishmania--cultivation and characterization of cultured extracellular amastigotes
Journal
Journal of Eukaryotic Microbiology
Author(s)
Pan  A. A., Duboise  S. M., Eperon  S., Rivas  L., Hodgkinson  V., Traub-Cseko  Y., McMahon-Pratt  D.
ISSN
1066-5234
Publication state
Published
Issued date
04/1993
Peer-reviewed
Oui
Volume
40
Number
2
Pages
213-23
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
Review --- Old month value: Mar-Apr
Abstract
The biochemistry and immunology of Leishmania promastigotes has been extensively studied; this is due primarily to the facility with which this stage, in contrast to the amastigotes stage, can be maintained in axenic culture. Several attempts to axenically culture lines of Leishmania amastigotes have been reported in the literature. This paper summarizes methods of adaptation (low pH, elevated temperature and culture medium) and characterization of several axenic lines of Leishmania amastigotes. Based on morphological, biological, immunological and biochemical evidence, these organisms appear to resemble amastigotes from infected macrophages or tissue. The axenically cultured amastigotes appear to be distinct from shocked (heat, serum deprivation, stressed) Leishmania promastigotes in the plethora of proteins synthesized, growth (multiplication) in culture, and developmental regulation observed. These data suggest that Leishmania organisms have a significant developmental response to certain signals (pH, temperature) mimicking their in vivo macrophage milieu. The response to other environmental parameters characteristic of the host-macrophage remain to be determined. These axenically cultured amastigotes should be of interest for further immunological, biochemical and developmental investigations of the disease-maintaining stage of this parasite.
Keywords
Adaptation, Physiological Animals Antibodies, Monoclonal Germ-Free Life Leishmania/*growth & development/immunology/ultrastructure Species Specificity
Pubmed
Web of science
Create date
17/01/2008 14:27
Last modification date
20/08/2019 14:27
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