Evolution of C(4) phosphoenolpyruvate carboxykinase in grasses, from genotype to phenotype.

Details

Serval ID
serval:BIB_37EBFB47DFC6
Type
Article: article from journal or magazin.
Collection
Publications
Title
Evolution of C(4) phosphoenolpyruvate carboxykinase in grasses, from genotype to phenotype.
Journal
Molecular Biology and Evolution
Author(s)
Christin P.A., Petitpierre B., Salamin N., Büchi L., Besnard G.
ISSN
1537-1719 (Electronic)
ISSN-L
0737-4038
Publication state
Published
Issued date
2009
Peer-reviewed
Oui
Volume
26
Number
2
Pages
357-365
Language
english
Abstract
C(4) photosynthesis is an adaptation over the classical C(3) pathway that has evolved multiple times independently. These convergences are accompanied by strong variations among the independent C(4) lineages. The decarboxylating enzyme used to release CO(2) around Rubisco particularly differs between C(4) species, a criterion used to distinguish three distinct biochemical C(4) subtypes. The phosphoenolpyruvate carboxykinase (PCK) serves as a primary decarboxylase in a minority of C(4) species. This enzyme is also present in C(3) plants, where it is responsible for nonphotosynthetic functions. The genetic changes responsible for the evolution of C(4)-specific PCK are still unidentified. Using phylogenetic analyses on PCK sequences isolated from C(3) and C(4) grasses, this study aimed at resolving the evolutionary history of C(4)-specific PCK enzymes. Four independent evolutions of C(4)-PCK were shown to be driven by positive selection, and nine C(4)-adaptive sites underwent parallel genetic changes in different C(4) lineages. C(4)-adaptive residues were also observed in C(4) species from the nicotinamide adenine dinucleotide phosphate-malic enzyme (NADP-ME) subtype and particularly in all taxa where a PCK shuttle was previously suggested to complement the NADP-ME pathway. Acquisitions of C(4)-specific PCKs were mapped on a species tree, which revealed that the PCK subtype probably appeared at the base of the Chloridoideae subfamily and was then recurrently lost and secondarily reacquired at least three times. Linking the genotype to subtype phenotype shed new lights on the evolutionary transitions between the different C(4) subtypes.
Keywords
Evolution, Molecular, Isoenzymes/genetics, Phosphoenolpyruvate Carboxylase/genetics, Phylogeny, Plant Proteins/genetics, Poaceae/classification, Poaceae/enzymology
Pubmed
Web of science
Open Access
Yes
Create date
18/11/2008 18:32
Last modification date
20/08/2019 14:26
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