Biocompatibility of bioresorbable poly(L-lactic acid) composite scaffolds obtained by supercritical gas foaming with human fetal bone cells

Details

Serval ID
serval:BIB_37D38690C71D
Type
Article: article from journal or magazin.
Collection
Publications
Title
Biocompatibility of bioresorbable poly(L-lactic acid) composite scaffolds obtained by supercritical gas foaming with human fetal bone cells
Journal
Tissue Engineering
Author(s)
Montjovent  M. O., Mathieu  L., Hinz  B., Applegate  L. L., Bourban  P. E., Zambelli  P. Y., Manson  J. A., Pioletti  D. P.
ISSN
1076-3279 (Print)
Publication state
Published
Issued date
12/2005
Volume
11
Number
11-12
Pages
1640-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov-Dec
Abstract
The aim of this investigation was to test the biocompatibility of three-dimensional bioresorbable foams made of poly(L-lactic acid) (PLA), alone or filled with hydroxyapatite (HA) or beta-tricalcium phosphate (beta-TCP), with human primary osteoblasts, using a direct contact method. Porous constructs were processed by supercritical gas foaming, after a melt-extrusion of ceramic/polymer mixture. Three neat polymer foams, with pore sizes of 170, 310, and 600 microm, and two composite foams, PLA/5 wt% HA and PLA/5 wt% beta-TCP, were examined over a 4-week culture period. The targeted application is the bone tissue-engineering field. For this purpose, human fetal and adult bone cells were chosen because of their highly osteogenic potential. The association of fetal bone cells and composite scaffold should lead to in vitro bone formation. The polymer and composite foams supported adhesion and intense proliferation of seeded cells, as revealed by scanning electron microscopy. Cell differentiation toward osteoblasts was demonstrated by alkaline phosphatase (ALP) enzymatic activity, gamma-carboxylated Gla-osteocalcin production, and the onset of mineralization. The addition of HA or beta-TCP resulted in higher ALP enzymatic activity for fetal bone cells and a stronger production of Gla-osteocalcin for adult bone cells.
Keywords
*Bone Substitutes *Calcium Phosphates Cells, Cultured Fetus/*physiology/ultrastructure Humans *Lactic Acid Materials Testing/methods Osteoblasts/*physiology/ultrastructure Osteogenesis/*physiology *Polymers Tissue Engineering/methods
Pubmed
Web of science
Create date
28/01/2008 12:29
Last modification date
20/08/2019 13:26
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