Induction of an immune response through the idiotypic network with monoclonal anti-idiotype antibodies in the carcinoembryonic antigen system.
Details
Serval ID
serval:BIB_37B1C6DBEB2C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Induction of an immune response through the idiotypic network with monoclonal anti-idiotype antibodies in the carcinoembryonic antigen system.
Journal
Journal of Cellular Biochemistry
ISSN
0730-2312 (Print)
ISSN-L
0730-2312
Publication state
Published
Issued date
1992
Peer-reviewed
Oui
Volume
50
Number
3
Pages
324-335
Language
english
Abstract
Anti-idiotype antibodies can mimic the conformational epitopes of the original antigen and act as antigen substitutes for vaccination and/or serological purposes. To investigate this possibility concerning the tumor marker carcinoembryonic antigen (CEA), BALB/c mice were immunized with the previously described anti-CEA monoclonal antibody (MAb) 5.D11 (AB1). After cell fusion, 15 stable cloned cell lines secreting anti-Ids (AB2) were obtained. Selected MAbs gave various degrees of inhibition (up to 100%) of the binding of 125I-labeled CEA to MAb 5.D11. Absence of reactivity of anti-Id MAbs with normal mouse IgG was first demonstrated by the fact that anti-Id MAbs were not absorbed by passage through a mouse IgG column, and second because they bound specifically to non-reduced MAb 5.D11 on Western blots. Anti-5.D11 MAbs did not inhibit binding to CEA of MAb 10.B9, another anti-CEA antibody obtained in the same fusion as 5.D11, or that of several anti-CEA MAbs reported in an international workshop, with the exception of two other anti-CEA MAbs, both directed against the GOLD IV epitope. When applied to an Id-anti-Id competitive radioimmunoassay, a sensitivity of 2 ng/ml of CEA was obtained, which is sufficient for monitoring circulating CEA in carcinoma patients. To verify that the anti-Id MAbs have the potential to be used as CEA vaccines, syngeneic BALB/c mice were immunized with these MAbs (AB2). Sera from immunized mice were demonstrated to contain AB3 antibodies recognizing the original antigen, CEA, both in enzyme immunoassay and by immunoperoxidase staining of human colon carcinoma. These results open the perspective of vaccination against colorectal carcinoma through the use of anti-idiotype antibodies as antigen substitutes.
Keywords
Animals, Antibodies, Anti-Idiotypic/immunology, Antibodies, Monoclonal/immunology, Antibody Formation, Antibody Specificity, Binding, Competitive, Carcinoembryonic Antigen/immunology, Epitopes, Immunoglobulin Idiotypes/immunology, Immunoglobulin Isotypes/immunology, Mice, Mice, Inbred BALB C
Pubmed
Web of science
Create date
25/01/2008 12:27
Last modification date
20/08/2019 14:26