Nodal stability determines signaling range.

Details

Serval ID
serval:BIB_37920
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Nodal stability determines signaling range.
Journal
Current Biology
Author(s)
Le Good J.A., Joubin K., Giraldez A.J., Ben-Haim N., Beck S., Chen Y., Schier A.F., Constam D.B.
ISSN
0960-9822
Publication state
Published
Issued date
2005
Volume
15
Number
1
Pages
31-36
Language
english
Notes
Old uritopublisher value: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15649361
Abstract
Secreted TGFbeta proteins of the Nodal family pattern the vertebrate body axes and induce mesoderm and endoderm . Nodal proteins can act as morphogens , but the mechanisms regulating their activity and signaling range are poorly understood. In particular, it has been unclear how inefficient processing or rapid turnover of the Nodal protein influences autocrine and paracrine signaling properties . Here, we evaluate the role of Nodal processing and stability in tissue culture and zebrafish embryos. Removal of the pro domain potentiates autocrine signaling but reduces Nodal stability and signaling range. Insertion of an N-glycosylation site present in several related TGFbeta proteins increases the stability of mature Nodal. The stabilized form of Nodal acts at a longer range than the wild-type form. These results suggest that increased proteolytic maturation of Nodal potentiates autocrine signaling, whereas increased Nodal stability extends paracrine signaling.
Keywords
Amino Acid Sequence, Animals, Autocrine Communication/physiology, Body Patterning/physiology, COS Cells, Cell Line, Cercopithecus aethiops, DNA Primers, Gene Expression, Humans, Immunoblotting, Molecular Sequence Data, Mutagenesis, Nodal Protein, Paracrine Communication/physiology, Protein Structure, Tertiary, Sequence Alignment, Sequence Analysis, DNA, Signal Transduction/physiology, Transfection, Transforming Growth Factor beta/metabolism, Zebrafish
Pubmed
Web of science
Open Access
Yes
Create date
19/11/2007 12:36
Last modification date
20/08/2019 13:26
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