Pancreas-specific deletion of beta-catenin reveals Wnt-dependent and Wnt-independent functions during development.

Details

Serval ID
serval:BIB_37905
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Pancreas-specific deletion of beta-catenin reveals Wnt-dependent and Wnt-independent functions during development.
Journal
Current Biology
Author(s)
Dessimoz J., Bonnard C., Huelsken J., Grapin-Botton A.
ISSN
0960-9822
Publication state
Published
Issued date
2005
Volume
15
Number
18
Pages
1677-1683
Language
english
Notes
Publication types: Comparative Study
Abstract
Mutations and deregulation of adenomatous polyposis coli (APC) and beta-catenin are implicated in specific cancers of the pancreas, but the role of Wnt pathway in normal pancreas development and homeostasis is unknown. This article reports a comprehensive investigation of the activity and the role of the Wnt pathway in pancreas organogenesis. We have used two reporter lines to monitor canonical Wnt pathway activity during development and after birth and demonstrate activity in endocrine cells and in the mesenchyme. We have specifically deleted the beta-catenin gene in the epithelium of the pancreas and duodenum by using Pdx1-Cre mice. In agreement with Wnt pathway activity in pancreatic endocrine cells, we find a reduction in endocrine islet numbers. Our study reveals that beta-catenin deletion also affects cells in which Wnt pathway activity is not detected. Indeed, beta-catenin mutant cells have a competitive disadvantage during development that also affects the exocrine compartment. Moreover, the conditional knockout (KO) mice develop acute edematous pancreatitis perinatally due to the disruption of the epithelial structure of acini. These effects are likely to be due to the function of beta-catenin at the membrane. Mice later recover from pancreatitis and regenerate normal pancreas and duodenal villi from the wild-type (wt) cells that escape beta-catenin deletion.
Keywords
Animals, Blood Glucose, Bromodeoxyuridine, Galactosides, Gene Deletion, Immunoblotting, Immunohistochemistry, Indoles, Mice, Mice, Knockout, Microscopy, Electron, Organogenesis/genetics, Organogenesis/physiology, Pancreas/embryology, Pancreas/ultrastructure, Signal Transduction/physiology, Wnt Proteins/metabolism, beta Catenin/genetics, beta-Galactosidase
Pubmed
Web of science
Open Access
Yes
Create date
19/11/2007 12:36
Last modification date
20/08/2019 13:26
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