A fetal sheep liver extract containing immunostimulatory substances including LPS acts as leukocyte activator in cells of LPS responder and non responder mice.

Details

Serval ID
serval:BIB_37777
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A fetal sheep liver extract containing immunostimulatory substances including LPS acts as leukocyte activator in cells of LPS responder and non responder mice.
Journal
International Immunopharmacology
Author(s)
Müller S., Hoffmann P., Esche U.V., Mach J.P., Gorczynski R.M., Waelli T., Alexander C., Zähringer U., Rietschel E.T., Bessler W.G., Westphal O.
ISSN
1567-5769 (Print)
ISSN-L
1567-5769
Publication state
Published
Issued date
2005
Peer-reviewed
Oui
Volume
5
Number
13-14
Pages
1809-1819
Language
english
Abstract
Purified fractions from a fetal sheep liver extract (FSLE) were investigated, in a murine model, for induction of leukocyte stimulating activities. The fractions FSLE-1 and FSLE-2 induced splenocyte proliferation in vitro in C57Bl/10ScSn (LPS responder) mice comparable to LPS, and in C57Bl/10ScCr (LPS non responder) mice. They also stimulated the release of nitrogen radicals in bone marrow-derived macrophages (BMDM) from several mouse inbred strains including both C57Bl/10ScSn and C57Bl/10ScCr mice. Stimulation of NO production could be blocked by L-NMMA, an inhibitor of iNOS, and enhanced by the simultaneous addition of IFN-gamma. Moreover, stimulation of macrophages by FSLE-1 and FSLE-2 induced a cytostatic effect of the activated macrophages for Abelson 8-1 tumor cells. The stimulatory activity of the purified fractions is partially due to trace amounts of LPS derived from the fetal liver extract which was enriched during purification. Our results may help to explain the beneficial effect of the extract in patients which has been observed clinically.
Keywords
Animals, Cell Line, Tumor/drug effects, Cell Line, Tumor/metabolism, Cell Proliferation/drug effects, Coculture Techniques, Dose-Response Relationship, Drug, Immunologic Factors/isolation & purification, Immunologic Factors/pharmacology, Interferon-gamma/pharmacology, Leukocytes/drug effects, Lipopolysaccharides/isolation & purification, Lipopolysaccharides/pharmacology, Liver/chemistry, Liver/embryology, Macrophages/drug effects, Macrophages/metabolism, Mice, Mice, Inbred C57BL, Nitric Oxide/metabolism, Nitric Oxide Synthase Type II/antagonists & inhibitors, Sheep/embryology, Spleen/cytology, Spleen/drug effects, omega-N-Methylarginine/pharmacology
Pubmed
Web of science
Create date
19/11/2007 13:36
Last modification date
20/08/2019 14:25
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