Preparation and analysis of fetal liver extracts.
Details
Serval ID
serval:BIB_376EA121579A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Preparation and analysis of fetal liver extracts.
Journal
Bone marrow transplantation
ISSN
0268-3369
Publication state
Published
Issued date
2000
Peer-reviewed
Oui
Volume
26
Number
6
Pages
667-71
Language
english
Notes
Publication types: Journal Article - Publication Status: ppublish
Abstract
The aim of this work is to describe the techniques that have been used for preparation and analysis of whole fetal liver extracts destined for in utero transplantation. Nine fetal livers between 12 and 17 weeks of gestation were prepared: cell counts and assessment of the hematopoietic cell viability were performed on cell suspensions. Hepatocytes represented 40 to 80% of the whole cell population. The remaining cells were constituted by hematopoietic cells (mainly erythroblasts), as well as by endothelial cells. The latter expressed CD34 on their surface, interfering with the assessment of CD34+ hematopoietic cells by flow cytometry. Direct visual morphologic control using alkaline phosphatase anti-alkaline phosphatase techniques was needed to differentiate hematopoietic from extra-hematopoietic CD34+ cells. Between 3.0 and 34.6 x 10(6) CD34+ viable hematopoietic cells were collected per fetal liver. Adequate differentiation of these cells into burst-forming units erythroid (BFU-E), colony-forming units granulocyte-macrophage (CFU-GM), and colony-forming units granulocyte erythroid macrophage megakaryocyte (CFU-GEMM) has been shown for each sample in clonogeneic cultures. In conclusion, fetal liver is a potential source of hematopoietic stem cells. Their numeration, based on the presence of CD34, is hampered by the expression of this antigen on other cells contained in the liver cell extract, in particular endothelial cells.
Keywords
Antigens, CD34, Cell Culture Techniques, Cell Differentiation, Cell Survival, Female, Hematopoietic Stem Cell Transplantation, Hematopoietic Stem Cells, Humans, Liver, Pregnancy, Tissue Extracts
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 16:34
Last modification date
20/08/2019 14:25