Insulin resistance is associated with elevated serum pigment epithelium-derived factor (PEDF) levels in morbidly obese patients.

Details

Serval ID
serval:BIB_374082E15249
Type
Article: article from journal or magazin.
Collection
Publications
Title
Insulin resistance is associated with elevated serum pigment epithelium-derived factor (PEDF) levels in morbidly obese patients.
Journal
Acta Diabetologica
Author(s)
Gattu A.K., Birkenfeld A.L., Jornayvaz F., Dziura J., Li F., Crawford S.E., Chu X., Still C.D., Gerhard G.S., Chung C., Samuel V.
ISSN
1432-5233 (Electronic)
ISSN-L
0940-5429
Publication state
Published
Issued date
2012
Peer-reviewed
Oui
Volume
49
Number
Suppl 1
Pages
S161-S169
Language
english
Abstract
Obesity is a significant risk factor for developing diabetes. Pigment epithelium-derived factor (PEDF) has been identified by experimental and clinical studies as both a causative and counter-regulatory factor in the metabolic syndrome. We set out to determine whether serum PEDF levels correlated with the degree of insulin resistance in morbidly obese patients. Sera from 53 patients who were evaluated prior to gastric bypass surgery were analyzed for PEDF levels using a commercial ELISA. None of the patients were on diabetes medications prior to enrollment. Baseline data included BMI, serum glucose and insulin, and homeostasis model assessment (HOMA) scores. Patients were stratified based on HOMA score and glucose levels into three groups: insulin sensitive (IS): HOMA <2 and glucose <126; insulin resistant (IR): HOMA >2 and glucose ≤126; and diabetes mellitus (DM): HOMA >2 and glucose >126. Pre- and post-gastric bypass sera from 12 patients were obtained for serial assessment of metabolic parameters and PEDF levels. PEDF secretion was assessed in primary human hepatocytes, HCC cells, and cultured adipocytes in the absence and presence of high glucose media. No significant differences in age, gender, and BMI were found among the three groups. PEDF levels were similar between IR patients and the other groups, but were significantly higher in DM compared to IS patients (p = 0.01). Serum PEDF in individual patients declined significantly after gastric bypass (p = 0.006). High glucose media led to significantly higher PEDF release by human hepatocytes in vitro (p = 0.016). These data demonstrate that serum PEDF concentrations better relate to insulin resistance than to adiposity and suggest that PEDF expression is closely linked to the development of insulin resistance.
Keywords
Adipocytes/metabolism, Adult, Blood Glucose/metabolism, Cells, Cultured, Eye Proteins/blood, Female, Humans, Insulin/therapeutic use, Insulin Resistance, Male, Middle Aged, Nerve Growth Factors/blood, Obesity, Morbid/blood, Obesity, Morbid/drug therapy, Serpins/blood
Pubmed
Web of science
Create date
10/09/2015 12:20
Last modification date
20/08/2019 13:25
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