Bacillus subtilis alpha-phosphoglucomutase is required for normal cell morphology and biofilm formation.

Details

Serval ID
serval:BIB_37324
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Bacillus subtilis alpha-phosphoglucomutase is required for normal cell morphology and biofilm formation.
Journal
Applied and Environmental Microbiology
Author(s)
Lazarevic V., Soldo B., Médico N., Pooley H., Bron S., Karamata D.
ISSN
0099-2240[print], 0099-2240[linking]
Publication state
Published
Issued date
2005
Volume
71
Number
1
Pages
39-45
Language
english
Abstract
Mutations designated gtaC and gtaE that affect alpha-phosphoglucomutase activity required for interconversion of glucose 6-phosphate and alpha-glucose 1-phosphate were mapped to the Bacillus subtilis pgcA (yhxB) gene. Backcrossing of the two mutations into the 168 reference strain was accompanied by impaired alpha-phosphoglucomutase activity in the soluble cell extract fraction, altered colony and cell morphology, and resistance to phages phi29 and rho11. Altered cell morphology, reversible by additional magnesium ions, may be correlated with a deficiency in the membrane glycolipid. The deficiency in biofilm formation in gtaC and gtaE mutants may be attributed to an inability to synthesize UDP-glucose, an important intermediate in a number of cell envelope biosynthetic processes.
Keywords
Bacillus Phages/physiology, Bacillus subtilis/enzymology, Bacillus subtilis/genetics, Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Base Sequence, Biofilms/growth & development, Gene Expression Regulation, Bacterial, Glucosephosphates/biosynthesis, Molecular Sequence Data, Mutation, Phosphoglucomutase/chemistry, Phosphoglucomutase/genetics
Pubmed
Web of science
Open Access
Yes
Create date
19/11/2007 12:35
Last modification date
20/08/2019 13:25
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