Superior late patency of small-diameter Dacron grafts seeded with omental microvascular cells: an experimental study

Details

Serval ID
serval:BIB_36BB66DAD861
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Superior late patency of small-diameter Dacron grafts seeded with omental microvascular cells: an experimental study
Journal
Annals of Thoracic Surgery
Author(s)
Pasic  M., Muller-Glauser  W., von Segesser  L. K., Lachat  M., Mihaljevic  T., Turina  M. I.
ISSN
0003-4975
Publication state
Published
Issued date
09/1994
Peer-reviewed
Oui
Volume
58
Number
3
Pages
677-684
Notes
Journal Article Research Support, Non-U.S. Gov't --- Old month value: Sep
Abstract
The purpose of the study was to investigate the effect of omental microvascular cell seeding on the patency of small-diameter Dacron prostheses usable for coronary artery bypass grafting. In a canine carotid artery model, each dog (n = 64) received one seeded and one similar nonseeded Dacron prosthesis (internal diameter = 4 or 6 mm). Enzymatically harvested omental microvascular cells (omentum = 27.6 +/- 5.9 g [+/- the standard deviation]; range, 17 to 50 g) were seeded prior to implantation. The seeding density was 1.91 +/- 0.26 [+/- the standard error] x 10(6) cells/cm2 of graft surface. Dipyridamole (75 mg/d) and acetylsalicylic acid (325 mg/d) were administered orally for 4 weeks postoperatively. The prostheses were explanted between 2 and 52 weeks after placement. The results were assessed by angiography; light, scanning electron, and transmission electron microscopy; and morphometry. The seeded grafts developed a uniform luminal monolayer of endothelial cells with minimal platelet or cellular deposition. These grafts also had a significantly higher overall patency rate and significantly larger thrombus-free surface areas than the nonseeded grafts. The overall actuarial patency rates at 1 week, 5, 12, 26, and 52 weeks were 100%, 98%, 93%, 93%, and 93%, respectively, for seeded Dacron grafts and 100%, 91%, 61%, 54%, and 18%, respectively, for nonseeded grafts. The patency rates of Dacron grafts usable for coronary artery bypass grafting are significantly improved by seeding with omental microvascular cells in a canine model.
Keywords
Actuarial Analysis Animals Blood Vessel Prosthesis/*instrumentation/methods Carotid Arteries/*surgery Cell Division *Cell Transplantation Coronary Artery Bypass/*instrumentation/methods Dogs Endothelium, Vascular/*cytology/physiology Graft Survival Microscopy, Electron, Scanning Models, Biological *Omentum *Polyethylene Terephthalates *Prostheses and Implants Prosthesis Design Surface Properties Time Factors *Vascular Patency
Pubmed
Web of science
Create date
14/02/2008 14:15
Last modification date
20/08/2019 13:24
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