Peripheral Nerve Repair: Multimodal Comparison of the Long-Term Regenerative Potential of Adipose Tissue-Derived Cells in a Biodegradable Conduit.

Details

Serval ID
serval:BIB_36AD43D1A3B8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Peripheral Nerve Repair: Multimodal Comparison of the Long-Term Regenerative Potential of Adipose Tissue-Derived Cells in a Biodegradable Conduit.
Journal
Stem Cells and Development
Author(s)
Kappos E.A., Engels P.E., Tremp M., Meyer zu Schwabedissen M., di Summa P., Fischmann A., von Felten S., Scherberich A., Schaefer D.J., Kalbermatten D.F.
ISSN
1557-8534 (Electronic)
ISSN-L
1547-3287
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
24
Number
18
Pages
2127-2141
Language
english
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Tissue engineering is a popular topic in peripheral nerve repair. Combining a nerve conduit with supporting adipose-derived cells could offer an opportunity to prevent time-consuming Schwann cell culture or the use of an autograft with its donor site morbidity and eventually improve clinical outcome. The aim of this study was to provide a broad overview over promising transplantable cells under equal experimental conditions over a long-term period. A 10-mm gap in the sciatic nerve of female Sprague-Dawley rats (7 groups of 7 animals, 8 weeks old) was bridged through a biodegradable fibrin conduit filled with rat adipose-derived stem cells (rASCs), differentiated rASCs (drASCs), human (h)ASCs from the superficial and deep abdominal layer, human stromal vascular fraction (SVF), or rat Schwann cells, respectively. As a control, we resutured a nerve segment as an autograft. Long-term evaluation was carried out after 12 weeks comprising walking track, morphometric, and MRI analyses. The sciatic functional index was calculated. Cross sections of the nerve, proximal, distal, and in between the two sutures, were analyzed for re-/myelination and axon count. Gastrocnemius muscle weights were compared. MRI proved biodegradation of the conduit. Differentiated rat ASCs performed significantly better than undifferentiated rASCs with less muscle atrophy and superior functional results. Superficial hASCs supported regeneration better than deep hASCs, in line with published in vitro data. The best regeneration potential was achieved by the drASC group when compared with other adipose tissue-derived cells. Considering the ease of procedure from harvesting to transplanting, we conclude that comparison of promising cells for nerve regeneration revealed that particularly differentiated ASCs could be a clinically translatable route toward new methods to enhance peripheral nerve repair.
Keywords
Absorbable Implants, Adipocytes/cytology, Adipose Tissue/cytology, Animals, Cell- and Tissue-Based Therapy/methods, Cells, Cultured, Female, Fibrin/metabolism, Models, Animal, Muscle, Skeletal/growth & development, Muscle, Skeletal/innervation, Nerve Regeneration/physiology, Neurogenesis/physiology, Peripheral Nerve Injuries/therapy, Rats, Rats, Sprague-Dawley, Sciatic Nerve/cytology, Sciatic Nerve/injuries, Stem Cell Transplantation/methods, Stem Cells/cytology, Tissue Engineering/methods
Pubmed
Web of science
Create date
29/09/2015 16:22
Last modification date
20/08/2019 13:24
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