DNA methyltransferase inhibitor alleviates bleomycin-induced pulmonary inflammation.

Details

Serval ID
serval:BIB_369221151E1A
Type
Article: article from journal or magazin.
Collection
Publications
Title
DNA methyltransferase inhibitor alleviates bleomycin-induced pulmonary inflammation.
Journal
International immunopharmacology
Author(s)
Lu C.H., Chen C.M., Ma J., Wu C.J., Chen L.C., Kuo M.L.
ISSN
1878-1705 (Electronic)
ISSN-L
1567-5769
Publication state
Published
Issued date
07/2020
Peer-reviewed
Oui
Volume
84
Pages
106542
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Acute lung injury (ALI) is a severe disease characterized by several inflammatory responses in the lung with high mortality. We applied a mouse model of the pulmonary inflammation induced by the intratracheal instillation of bleomycin which is widely used to induce ALI and fibrosis in animal models. We hypothesized that DNA methyltransferase inhibitor, 5-azacytidine (5-Aza), with its anti-inflammatory benefits, might attenuate bleomycin-induced ALI through the alleviation of inflammation in the lung. We quantified white blood cells with cell blood count (CBC) test, lung inflammation by analyzing cells in the collected bronchoalveolar lavage fluid (BALF) and histological analysis of the lung tissues, and gene expression levels by real-time PCR. Intratracheal administration of bleomycin in mice induced pulmonary inflammation, characterized by increased neutrophil infiltration and inflammatory cytokine expression in the lungs. Mice treated with 5-Aza showed a significant reduction of lung neutrophilia, together with lower expressions of CXCL2 and MCP-1. Furthermore, 5-Aza treatment decreased the expression of proinflammatory cytokines in the lung tissue. Collectively, our data show that DNA methyltransferase inhibitor can alleviate the lung inflammation of bleomycin-induced ALI, indicating an alternative treatment option for the inflammation-triggered lung injury.
Keywords
Acute lung inflammation, Bleomycin, DNA methyltransferase inhibitor, Neutrophils, Pulmonary inflammation
Pubmed
Create date
16/06/2020 15:14
Last modification date
11/01/2021 9:14
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