PET CMR<sub>glc</sub> mapping and <sup>1</sup>H-MRS show altered glucose uptake and neurometabolic profiles in BDL rats.

Details

Ressource 1Request a copy Under indefinite embargo.
UNIL restricted access
State: Public
Version: author
License: CC BY-NC-ND 4.0
Serval ID
serval:BIB_36646523A3F6
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
PET CMR<sub>glc</sub> mapping and <sup>1</sup>H-MRS show altered glucose uptake and neurometabolic profiles in BDL rats.
Journal
Analytical biochemistry
Author(s)
Mosso J., Yin T., Poitry-Yamate C., Simicic D., Lepore M., McLin V.A., Braissant O., Cudalbu C., Lanz B.
ISSN
1096-0309 (Electronic)
ISSN-L
0003-2697
Publication state
Published
Issued date
15/06/2022
Peer-reviewed
Oui
Volume
647
Pages
114606
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Type C hepatic encephalopathy (HE) is a complex neuropsychiatric disorder occurring as a consequence of chronic liver disease. Alterations in energy metabolism have been suggested in type C HE, but in vivo studies on this matter remain sparse and have reported conflicting results. Here, we propose a novel preclinical <sup>18</sup> F-FDG PET methodology to compute quantitative 3D maps of the regional cerebral metabolic rate of glucose (CMR <sub>glc</sub> ) from a labelling steady-state PET image of the brain and an image-derived input function. This quantitative approach shows its strength when comparing groups of animals with divergent physiology, such as HE animals. PET CMR <sub>glc</sub> maps were registered to an atlas and the mean CMR <sub>glc</sub> from the hippocampus and the cerebellum were associated to the corresponding localized <sup>1</sup> H MR spectroscopy acquisitions. This study provides for the first time local and quantitative information on both brain glucose uptake and neurometabolic profile alterations in a rat model of type C HE. A 2-fold lower brain glucose uptake, concomitant with an increase in brain glutamine and a decrease in the main osmolytes, was observed in the hippocampus and in the cerebellum. These novel findings are an important step towards new insights into energy metabolism in the pathophysiology of HE.
Keywords
Animals, Brain/diagnostic imaging, Brain/metabolism, Glucose/metabolism, Glutamine/metabolism, Hepatic Encephalopathy/metabolism, Proton Magnetic Resonance Spectroscopy, Rats, (1)H MRS, (18)F-FDG PET, Bile duct ligation, Cerebral metabolic rate of glucose, Rat brain, Type C hepatic encephalopathy
Pubmed
Open Access
Yes
Create date
14/03/2022 10:08
Last modification date
20/07/2022 6:39
Usage data