Expression of prox1, lymphatic endothelial nuclear transcription factor, in Kaposiform hemangioendothelioma and tufted angioma.

Details

Serval ID
serval:BIB_3651F4DE90D4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Expression of prox1, lymphatic endothelial nuclear transcription factor, in Kaposiform hemangioendothelioma and tufted angioma.
Journal
American Journal of Surgical Pathology
Author(s)
Le Huu Aude Rimella, Jokinen Chris H., Ruben Brian P., Mihm Martin C., Weiss Sharon W., North Paula E., Dadras Soheil S.
ISSN
1532-0979[electronic], 0147-5185[linking]
Publication state
Published
Issued date
2010
Volume
34
Number
11
Pages
1563-1573
Language
english
Abstract
Kaposiform hemangioendothelioma (KHE) and tufted angioma (TA) are rare tumors mainly occurring in early childhood. Our recent results showed that ectopic overexpression of human Prox1 gene, a lymphatic endothelial nuclear transcription factor, promoted an aggressive behavior in 2 murine models of KHE. This dramatic Prox1-induced phenotype prompted us to investigate immunohistochemical staining pattern of Prox1, podoplanin (D2-40), LYVE-1, and Prox1/CD34 as well as double immunofluorescent staining pattern of LYVE-1/CD31 in KHE and TA, compared with other pediatric vascular tumors. For this purpose, we examined 75 vascular lesions: KHE (n=18), TA (n=13), infantile hemangioma (n=13), pyogenic granuloma (n=18), and granulation tissue (n=13). Overall, KHE and TA shared an identical endothelial immunophenotype: the neoplastic spindle cells were Prox1, podoplanin, LYVE-1, CD31, and CD34, whereas endothelial cells within glomeruloid foci were Prox1, podoplanin, LYVE-1, CD31, and CD34. The lesional cells of all infantile hemangiomas and pyogenic granulomas were negative for Prox1 in the presence of positive internal control. These findings provide immunophenotypic evidence to support a preexisting notion that KHE and TA are closely related, if not identical. Overall, our results show, for the first time, that Prox1 is an immunohistochemical biomarker helpful in confirming the diagnosis of KHE/TA and in distinguishing it from infantile hemangioma and pyogenic granuloma.
Keywords
Adolescent, Antigens, CD31/analysis, Antigens, CD34/analysis, Cell Nucleus/chemistry, Child, Child, Preschool, Cytoplasm/chemistry, Diagnosis, Differential, Granuloma, Pyogenic/metabolism, Hemangioendothelioma/chemistry, Hemangioendothelioma/classification, Hemangioma/chemistry, Hemangioma/classification, Homeodomain Proteins/analysis, Humans, Immunohistochemistry, Immunophenotyping, Infant, Membrane Glycoproteins/analysis, Predictive Value of Tests, Skin Neoplasms/chemistry, Skin Neoplasms/classification, Soft Tissue Neoplasms/chemistry, Soft Tissue Neoplasms/classification, Tumor Markers, Biological/analysis, Tumor Suppressor Proteins/analysis, Vesicular Transport Proteins/analysis
Pubmed
Web of science
Create date
19/11/2010 9:09
Last modification date
20/08/2019 13:24
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