The RRE of human immunodeficiency virus type 1 contributes to cell-type-specific viral tropism

Details

Serval ID
serval:BIB_36374A6A9689
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The RRE of human immunodeficiency virus type 1 contributes to cell-type-specific viral tropism
Journal
Journal of Virology
Author(s)
Dayton  E. T., Konings  D. A., Lim  S. Y., Hsu  R. K., Butini  L., Pantaleo  G., Dayton  A. I.
ISSN
0022-538X (Print)
Publication state
Published
Issued date
05/1993
Volume
67
Number
5
Pages
2871-8
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Abstract
As part of a general program investigating the mechanism of the Rev axis of human immunodeficiency virus type 1 (HIV-1) autoregulation, a series of proviral HIV-1 mutants which differ from the parental HXB2 strain at selected positions within the RRE were constructed. All of the mutations were designed to perturb the RRE by introducing local helix disruptions without altering the coding potential of the overlapping envelope open reading frame. Viral replication in various cell types was monitored by a cell supernatant reverse transcriptase assay and Northern (RNA blot) analysis. All proviral RRE mutants displayed at least some impairment in replication. However, the relative impairment varied drastically among the various cell types tested. This suggests that the RRE may contribute to cell-type-specific viral tropism.
Keywords
Acquired Immunodeficiency Syndrome/*microbiology Base Sequence Cell Line/microbiology Databases, Factual Gene Products, rev/metabolism HIV-1/genetics/growth & development/*pathogenicity Lymphocyte Activation Molecular Sequence Data Mutagenesis Nucleic Acid Conformation Proviruses/genetics/growth & development/*pathogenicity RNA, Viral/*genetics Sequence Alignment Virion/isolation & purification Virus Replication
Pubmed
Web of science
Create date
25/01/2008 15:13
Last modification date
20/08/2019 13:24
Usage data