Heterogeneous secretion of individual B cells in response to D-glucose and to nonglucidic nutrient secretagogues.

Details

Serval ID
serval:BIB_36343845B9AE
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Heterogeneous secretion of individual B cells in response to D-glucose and to nonglucidic nutrient secretagogues.
Journal
American Journal of Physiology
Author(s)
Bosco D., Meda P., Thorens B., Malaisse W.J.
ISSN
0363-6143
Publication state
Published
Issued date
03/1995
Volume
268
Number
3 Pt 1
Pages
C611-C618
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
We used a hemolytic plaque assay for insulin to determine whether the same pancreatic B cells respond to D-glucose, 2-amino-bicyclo[2,2,1]heptane-2-carboxylic acid (BCH) and the association of this nonmetabolized analogue of L-leucine with either the monomethyl ester of succinic acid (SME) or the dimethyl ester of L-glutamic acid (GME). During a 30-min incubation in the absence of D-glucose, BCH alone (5 mM) had no effect on insulin release. In contrast, the combination of BCH with either SME (10 mM) or GME (3 mM) stimulated insulin release to the same extent observed in the sole presence of 16.7 mM D-glucose. The effects of BCH plus SME and BCH plus GME on both percentage of secreting B cells and total insulin output were little affected in the presence of D-glucose concentrations ranging from 0 to 16.7 mM. Varying the concentration of SME from 2 to 10 mM also did not influence these effects. In other experiments, the very same B cells were first exposed 45 min to 16.7 mM D-glucose, then incubated 45 min in the presence of only BCH and SME. Under these conditions, most (80.3 +/- 2.5%) of the cells contributing to insulin release did so during both incubation periods. Furthermore, virtually all cells responding to BCH and SME during the second incubation corresponded to cells also responsive to D-glucose during the first incubation. Similar observations were made when the sequence of the two incubations was reversed.(ABSTRACT TRUNCATED AT 250 WORDS)
Keywords
Amino Acids/pharmacology, Amino Acids, Cyclic, Animals, DNA-Binding Proteins/genetics, Early Growth Response Protein 1, Glucokinase/metabolism, Glucose/pharmacology, Glucose Transporter Type 2, Glutamates/pharmacology, Hemolytic Plaque Technique, Immediate-Early Proteins, Insulin/secretion, Islets of Langerhans/drug effects, Islets of Langerhans/secretion, Leucine/analogs &amp, derivatives, Male, Monosaccharide Transport Proteins/metabolism, Rats, Rats, Sprague-Dawley, Succinates/pharmacology, Transcription Factors/genetics
Pubmed
Web of science
Create date
24/01/2008 13:41
Last modification date
20/08/2019 13:24
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