Envelope glycoproteins sampling states 2/3 are susceptible to ADCC by sera from HIV-1-infected individuals.
Details
Serval ID
serval:BIB_36097CF92206
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Envelope glycoproteins sampling states 2/3 are susceptible to ADCC by sera from HIV-1-infected individuals.
Journal
Virology
ISSN
1096-0341 (Electronic)
ISSN-L
0042-6822
Publication state
Published
Issued date
02/2018
Peer-reviewed
Oui
Volume
515
Pages
38-45
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
Recent analysis of HIV-1 envelope glycoproteins (Env) dynamics showed that the unliganded Env trimer can potentially sample three conformations: a metastable "closed" conformation (State 1), an "open" CD4-bound conformation (State 3), and an intermediate "partially open" conformation (State 2). HIV-1 evolved several mechanisms to avoid "opening" its Env in order to evade immune responses such as antibody-dependent cellular cytotoxicity (ADCC), which preferentially targets Envs in the CD4-bound conformation on the surface of infected cells. Here we took advantage of a well-characterized single-residue change in the gp120 trimer association domain to modify Env conformation and evaluate its impact on ADCC responses. We found that cells infected with viruses expressing Env stabilized in States 2/3 become highly susceptible to ADCC responses by sera from HIV-1-infected individuals. Our results indicate that the conformations spontaneously sampled by the Env trimer at the surface of infected cells has a significant impact on ADCC responses.
Keywords
Antibody-Dependent Cell Cytotoxicity, CD4 Antigens/immunology, HIV Antibodies/immunology, HIV Infections/immunology, HIV Infections/virology, HIV-1/genetics, HIV-1/immunology, HIV-1/physiology, Humans, Serum/immunology, env Gene Products, Human Immunodeficiency Virus/genetics, env Gene Products, Human Immunodeficiency Virus/immunology, ADCC, CD4, Envelope glycoproteins, HIV-1, Non-neutralizing antibodies, States 2/3
Pubmed
Web of science
Publisher's website
Open Access
Yes
Create date
09/05/2023 13:59
Last modification date
29/11/2024 17:54