Generation of procoagulant collagen- and thrombin-activated platelets in platelet concentrates derived from buffy coat: the role of processing, pathogen inactivation, and storage.

Details

Serval ID
serval:BIB_35EF9E8EF671
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Generation of procoagulant collagen- and thrombin-activated platelets in platelet concentrates derived from buffy coat: the role of processing, pathogen inactivation, and storage.
Journal
Transfusion
Author(s)
Bertaggia Calderara D., Crettaz D., Aliotta A., Barelli S., Tissot J.D., Prudent M., Alberio L.
ISSN
1537-2995 (Electronic)
ISSN-L
0041-1132
Publication state
Published
Issued date
10/2018
Peer-reviewed
Oui
Volume
58
Number
10
Pages
2395-2406
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Collagen- and thrombin-activated (COAT) platelets (PLTs), generated by dual-agonist stimulation with collagen and thrombin (THR), enhance THR generation at the site of vessel wall injury. There is evidence that higher amounts of procoagulant COAT PLTs are associated with stroke, while a decreased ability to generate them is associated with bleeding diathesis. Our aim was to study PLT functions, particularly the ability to generate COAT PLTs, in PLT concentrates (PCs) from buffy coat. Thus, we investigated the effect of processing, pathogen inactivation treatment (amotosalen-UVA), and PC storage.
Two PCs from five donors each were pooled and split in two bags; one of them was pathogen inactivated and the other one was left untreated (n = 5). Flow cytometric analyses were performed immediately after PC preparation (Day 1) and thereafter on Days 2, 5, 7, and 9 in treated and untreated PCs to measure the reactivity of PLTs (CD62P and PAC-1), the content and secretion of dense granule after stimulation with different agonists, and the percentage of COAT PLTs after dual stimulation with convulxin (agonist of the collagen receptor GPVI) and THR.
Preparation of PCs resulted in a significant decrease of COAT PLTs and in an impaired response to adenosine 5'-diphosphate sodium (ADP). Storage further decreased ADP response. Minor differences were observed between untreated or amotosalen-UVA-treated PCs.
Preparation of PCs from buffy coats decreased the ability to generate COAT PLTs and impaired PLT response to ADP.
Keywords
Adenosine Diphosphate/pharmacology, Blood Buffy Coat/cytology, Blood Platelets/cytology, Blood Preservation/methods, Collagen/pharmacology, Furocoumarins, Humans, Platelet Activation/drug effects, Sterilization/methods, Thrombin/pharmacology, Ultraviolet Rays
Pubmed
Web of science
Create date
25/09/2018 6:50
Last modification date
18/05/2020 13:47
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