The transcriptomic signature of responses to larval crowding in Drosophila melanogaster.

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State: Public
Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_35BCED98ABBA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The transcriptomic signature of responses to larval crowding in Drosophila melanogaster.
Journal
Insect science
Author(s)
Morimoto J., Wenzel M., Derous D., Henry Y., Colinet H.
ISSN
1744-7917 (Electronic)
ISSN-L
1672-9609
Publication state
Published
Issued date
04/2023
Peer-reviewed
Oui
Volume
30
Number
2
Pages
539-554
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Intraspecific competition at the larval stage is an important ecological factor affecting life-history, adaptation and evolutionary trajectory in holometabolous insects. However, the molecular pathways underpinning these ecological processes are poorly characterized. We reared Drosophila melanogaster at three egg densities (5, 60, and 300 eggs/mL) and sequenced the transcriptomes of pooled third-instar larvae. We also examined emergence time, egg-to-adult viability, adult mass, and adult sex-ratio at each density. Medium crowding had minor detrimental effects on adult phenotypes compared to low density and yielded 24 differentially expressed genes (DEGs), including several chitinase enzymes. In contrast, high crowding had substantial detrimental effects on adult phenotypes and yielded 2107 DEGs. Among these, upregulated gene sets were enriched in sugar, steroid and amino acid metabolism as well as DNA replication pathways, whereas downregulated gene sets were enriched in ABC transporters, taurine, Toll/Imd signaling, and P450 xenobiotics metabolism pathways. Overall, our findings show that larval crowding has a large consistent effect on several molecular pathways (i.e., core responses) with few pathways displaying density-specific regulation (i.e., idiosyncratic responses). This provides important insights into how holometabolous insects respond to intraspecific competition during development.
Keywords
Animals, Drosophila melanogaster/genetics, Larva, Transcriptome, Crowding, Phenotype, intraspecific competition, larval crowding, life-history, trade-offs, transcriptomics
Pubmed
Web of science
Open Access
Yes
Create date
27/09/2022 10:21
Last modification date
19/07/2023 7:09
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