Determination of zaleplon and zolpidem by liquid chromatography-turbo-ionspray mass spectrometry: application to forensic cases.

Details

Serval ID
serval:BIB_35691108F41A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Determination of zaleplon and zolpidem by liquid chromatography-turbo-ionspray mass spectrometry: application to forensic cases.
Journal
Journal of Chromatography. B, Analytical technologies in the biomedical and life sciences
Author(s)
Giroud C., Augsburger M., Menetrey A., Mangin P.
ISSN
1570-0232
Publication state
Published
Issued date
2003
Peer-reviewed
Oui
Volume
789
Number
1
Pages
131-138
Language
english
Abstract
Zolpidem and zaleplon are two short-acting hypnotic agents used in Europe and in the USA. An atmospheric pressure ionisation liquid chromatography-mass spectrometry (Sciex API 150 EX) method was developed for the determination of zolpidem and zaleplon in whole blood. After single-step liquid-liquid extraction, the hypnotics were separated by gradient-elution with an ammonium formate buffer/acetonitrile eluent on an Inertsil ODS-3 column. Methaqualone was used as internal standard. The recovery was higher than 70% for both hypnotics and the internal standard. The best fit for the calibration curve was achieved, between 1 and 250 ng/ml, with 1/x quadratic regression. Coefficients of intra- and inter-assay variation calculated at 5, 25 and 100 ng/ml were less than 10%. The method was successfully applied to forensic cases.
Keywords
Acetamides, Chromatography, Liquid, Forensic Medicine, Humans, Hypnotics and Sedatives, Mass Spectrometry, Pyridines, Pyrimidines, Reference Standards, Reproducibility of Results
Pubmed
Web of science
Create date
08/02/2008 17:43
Last modification date
20/08/2019 13:22
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