In Silico and Structural Analyses Demonstrate That Intrinsic Protein Motions Guide T Cell Receptor Complementarity Determining Region Loop Flexibility.

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Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_3562FF817297
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
In Silico and Structural Analyses Demonstrate That Intrinsic Protein Motions Guide T Cell Receptor Complementarity Determining Region Loop Flexibility.
Journal
Frontiers in immunology
Author(s)
Holland C.J., MacLachlan B.J., Bianchi V., Hesketh S.J., Morgan R., Vickery O., Bulek A.M., Fuller A., Godkin A., Sewell A.K., Rizkallah P.J., Wells S., Cole D.K.
ISSN
1664-3224 (Print)
ISSN-L
1664-3224
Publication state
Published
Issued date
2018
Peer-reviewed
Oui
Volume
9
Pages
674
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
T-cell immunity is controlled by T cell receptor (TCR) binding to peptide major histocompatibility complexes (pMHCs). The nature of the interaction between these two proteins has been the subject of many investigations because of its central role in immunity against pathogens, cancer, in autoimmunity, and during organ transplant rejection. Crystal structures comparing unbound and pMHC-bound TCRs have revealed flexibility at the interaction interface, particularly from the perspective of the TCR. However, crystal structures represent only a snapshot of protein conformation that could be influenced through biologically irrelevant crystal lattice contacts and other factors. Here, we solved the structures of three unbound TCRs from multiple crystals. Superposition of identical TCR structures from different crystals revealed some conformation differences of up to 5 Å in individual complementarity determining region (CDR) loops that are similar to those that have previously been attributed to antigen engagement. We then used a combination of rigidity analysis and simulations of protein motion to reveal the theoretical potential of TCR CDR loop flexibility in unbound state. These simulations of protein motion support the notion that crystal structures may only offer an artifactual indication of TCR flexibility, influenced by crystallization conditions and crystal packing that is inconsistent with the theoretical potential of intrinsic TCR motions.

Keywords
T cell receptor, T-cells, X-ray crystallography, complementarity determining regions loops, computational simulations, protein flexibility
Pubmed
Web of science
Open Access
Yes
Create date
30/04/2018 16:29
Last modification date
20/08/2019 13:22
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