Functionalization of microstructured open-porous bioceramic scaffolds with human fetal bone cells.

Details

Serval ID
serval:BIB_34C8474D9405
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Functionalization of microstructured open-porous bioceramic scaffolds with human fetal bone cells.
Journal
Bioconjugate Chemistry
Author(s)
Krauss Juillerat F., Borcard F., Staedler D., Scaletta C., Applegate L.A., Comas H., Gauckler L.J., Gerber-Lemaire S., Juillerat-Jeanneret L., Gonzenbach U.T.
ISSN
1520-4812 (Electronic)
ISSN-L
1043-1802
Publication state
Published
Issued date
2012
Volume
23
Number
11
Pages
2278-2290
Language
english
Notes
Publication types: Journal ArticlePublication Status: ppublish
Abstract
Bone substitute materials allowing trans-scaffold migration and in-scaffold survival of human bone-derived cells are mandatory for development of cell-engineered permanent implants to repair bone defects. In this study, we evaluated the influence on human bone-derived cells of the material composition and microstructure of foam scaffolds of calcium aluminate. The scaffolds were prepared using a direct foaming method allowing wide-range tailoring of the microstructure for pore size and pore openings. Human fetal osteoblasts (osteo-progenitors) attached to the scaffolds, migrated across the entire bioceramic depending on the scaffold pore size, colonized, and survived in the porous material for at least 6 weeks. The long-term biocompatibility of the scaffold material for human bone-derived cells was evidenced by in-scaffold determination of cell metabolic activity using a modified MTT assay, a repeated WST-1 assay, and scanning electron microscopy. Finally, we demonstrated that the osteo-progenitors can be covalently bound to the scaffolds using biocompatible click chemistry, thus enhancing the rapid adhesion of the cells to the scaffolds. Therefore, the different microstructures of the foams influenced the migratory potential of the cells, but not cell viability. Scaffolds allow covalent biocompatible chemical binding of the cells to the materials, either localized or widespread integration of the scaffolds for cell-engineered implants.
Pubmed
Create date
22/11/2012 11:19
Last modification date
20/08/2019 14:21
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