Splice variant-specific stabilization of JNKs by IB1/JIP1.

Details

Serval ID
serval:BIB_34A390AC6694
Type
Article: article from journal or magazin.
Collection
Publications
Title
Splice variant-specific stabilization of JNKs by IB1/JIP1.
Journal
Cellular signalling
Author(s)
Yang J.Y., Moulin N., van Bemmelen M.X., Dubuis G., Tawadros T., Haefliger J.A., Waeber G., Widmann C.
ISSN
0898-6568
Publication state
Published
Issued date
2007
Peer-reviewed
Oui
Volume
19
Number
10
Pages
2201-7
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Abstract
Islet-Brain 1 (IB1) (also called JNK-interacting protein 1; JIP1) is a scaffold protein that tethers components of the JNK mitogen-activated protein kinase pathway inducing a modulation of the activity and the target specificity of the JNK kinases. Dysfunctions in IB1 have been associated with diseases such as early type II diabetes. To gain more insight in the functions of IB1, its ability to modulate the expression levels of the various JNK proteins was assessed. Each of the three JNK genes gives rise to several splice variants encoding short or long proteins. The expression levels of the short JNK proteins, but not of the long variants, were systematically higher in rat tissues and in transformed cell lines expressing high IB1 levels compared to tissues and cells with no or low IB1 expression. HEK293 cells bearing a tetracycline-inducible IB1 construct showed a specific increase of the short JNK endogenous splice variants in the presence of tetracycline. The augmented expression level of the short JNK splice variants induced by IB1 resulted from an increased stability towards degradation. Modulation of the stability of specific JNK splice variants represents therefore a newly identified mechanism used by IB1 to regulate the JNK MAPK pathway.
Keywords
Adaptor Proteins, Signal Transducing, Alternative Splicing, Animals, Cell Line, Cell Line, Transformed, Enzyme Stability, Humans, JNK Mitogen-Activated Protein Kinases, Mitogen-Activated Protein Kinase 8, Mitogen-Activated Protein Kinase 9, Rats
Pubmed
Web of science
Create date
17/11/2008 8:57
Last modification date
20/08/2019 13:21
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