Large TCR diversity of virus-specific CD8 T cells provides the mechanistic basis for massive TCR renewal after antigen exposure.

Details

Serval ID
serval:BIB_34994BDAB209
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Large TCR diversity of virus-specific CD8 T cells provides the mechanistic basis for massive TCR renewal after antigen exposure.
Journal
Journal of immunology
Author(s)
Miconnet I., Marrau A., Farina A., Taffé P., Vigano S., Harari A., Pantaleo G.
ISSN
1550-6606 (Electronic)
ISSN-L
0022-1767
Publication state
Published
Issued date
15/06/2011
Peer-reviewed
Oui
Volume
186
Number
12
Pages
7039-7049
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Ex vivo analysis of virus-specific CD8 T cell populations by anchored PCR has shown that the CD8 TCR repertoire was less oligoclonal (seven to nine clonotypes per individual epitope) than previously thought. In the current study, TCR diversity was investigated by assessing both the overall TCR β-chain variable regions usage as well as the CDR3 regions in ex vivo-isolated CMV- and EBV-specific CD8 T cells from 27 healthy donors. The average number of clonotypes specific to most single viral epitopes comprised between 14 and 77. Changes in the CD8 TCR repertoire were also longitudinally assessed under conditions of HIV-1 chronic infection (i.e., in patients with suppressed virus replication and after treatment interruption and Ag re-exposure). The results showed that a large renewal (≤ 80%) of the TRB repertoire occurred after Ag re-exposure and was eventually associated with an increased T cell recognition functional avidity. These results demonstrate that the global CD8 TCR repertoire is much more diverse (≤ 9-fold) than previously estimated and provide the mechanistic basis for supporting massive repertoire renewal during chronic virus infection and Ag re-exposure.
Keywords
Antigenic Variation/immunology, Antigens, Viral/immunology, CD8-Positive T-Lymphocytes/immunology, CD8-Positive T-Lymphocytes/virology, Cells, Cultured, Cytomegalovirus/immunology, Herpesvirus 4, Human/immunology, Humans, Receptors, Antigen, T-Cell/immunology, T-Cell Antigen Receptor Specificity/immunology, Virus Diseases/immunology
Pubmed
Web of science
Open Access
Yes
Create date
17/08/2016 11:36
Last modification date
30/01/2020 7:26
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