Identification of a seven glycopeptide signature for malignant pleural mesothelioma in human serum by selected reaction monitoring.

Details

Serval ID
serval:BIB_348BE8B7ABC0
Type
Article: article from journal or magazin.
Collection
Publications
Title
Identification of a seven glycopeptide signature for malignant pleural mesothelioma in human serum by selected reaction monitoring.
Journal
Clinical Proteomics
Author(s)
Cerciello F., Choi M., Nicastri A., Bausch-Fluck D., Ziegler A., Vitek O., Felley-Bosco E., Stahel R., Aebersold R., Wollscheid B.
ISSN
1542-6416 (Print)
ISSN-L
1542-6416
Publication state
Published
Issued date
2013
Peer-reviewed
Oui
Volume
10
Number
1
Pages
16
Language
english
Notes
Publication types: Journal Article
Abstract
BACKGROUND: Serum biomarkers can improve diagnosis and treatment of malignant pleural mesothelioma (MPM). However, the evaluation of potential new serum biomarker candidates is hampered by a lack of assay technologies for their clinical evaluation. Here we followed a hypothesis-driven targeted proteomics strategy for the identification and clinical evaluation of MPM candidate biomarkers in serum of patient cohorts.
RESULTS: Based on the hypothesis that cell surface exposed glycoproteins are prone to be released from tumor-cells to the circulatory system, we screened the surfaceome of model cell lines for potential MPM candidate biomarkers. Selected Reaction Monitoring (SRM) assay technology allowed for the direct evaluation of the newly identified candidates in serum. Our evaluation of 51 candidate biomarkers in the context of a training and an independent validation set revealed a reproducible glycopeptide signature of MPM in serum which complemented the MPM biomarker mesothelin.
CONCLUSIONS: Our study shows that SRM assay technology enables the direct clinical evaluation of protein-derived candidate biomarker panels for which clinically reliable ELISA's currently do not exist.
Pubmed
Open Access
Yes
Create date
03/08/2014 11:13
Last modification date
20/08/2019 13:21
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