Multicenter evaluation of the Elecsys Toxo IgG and IgM tests for the diagnosis of infection with Toxoplasma gondii

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Version: Final published version
Serval ID
serval:BIB_346A4EF7CAFC
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Multicenter evaluation of the Elecsys Toxo IgG and IgM tests for the diagnosis of infection with Toxoplasma gondii
Journal
European Journal of Microbiology and Immunology
Author(s)
Meylan , Paris , Liesenfeld 
ISSN
2062-509X (Print)
2062-8633 (Electronic)
ISSN-L
2062-509X
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
5
Number
2
Pages
150-158
Language
english
Notes
Publication types: Original Article ; research-article Identifiant PubMed Central: PMC4500066
Abstract
Detection of IgG and IgM antibodies is commonly performed for the diagnosis of infection with Toxoplasma gondii. We determined the accuracy of the Elecsys Toxo IgG and IgM test at four European laboratories compared to local reference methods. Coefficients of variation for reproducibility ranged from 1.0 to 6.5% for IgG and from 0.8 to 3.2% for IgM. Seroconversion panels revealed high overall concordance with the reference tests. The Elecsys test detected IgG antibodies earlier than the Cobas Core IgG test in 19 of 47 panels; persisting IgM antibodies were observed in the VIDAS but not the Elecsys test in five of 47 panels. In 31.4% of latent stage sera with persistent IgM antibodies (positive LIASON IgM), the Elecsys IgM test gave negative results indicating increased "clinical" specificity. Sensitivity and specificity of the Elecsys IgG assay ranged from 99.45 to 100% and 87.50-99.80%, respectively, and 91.11-95.74 and 98.45-99.79% for the Elecsys IgM assay, respectively. In conclusion, excellent reproducibility and accuracy make the Elecsys Toxo G and M tests highly suitable for the detection of anti-T. gondii IgG and IgM antibodies. The lower detection rates for persistent IgM in the Elecsys IgM test increase "clinical" specificity and decrease the need for follow-up testing.
Keywords
Toxoplasma gondii, antibodies, IgM, IgG, seroconversion, latent infection
Pubmed
Web of science
Open Access
Yes
Create date
11/10/2016 15:29
Last modification date
20/08/2019 13:21
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