Molecular and functional diversity at synapses of individual neurons in vitro.

Details

Serval ID
serval:BIB_344
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Molecular and functional diversity at synapses of individual neurons in vitro.
Journal
European Journal of Neuroscience
Author(s)
Staple J.K., Osen-Sand A., Benfenati F., Pich E.M., Catsicas S.
ISSN
0953-816X (Print)
ISSN-L
0953-816X
Publication state
Published
Issued date
1997
Volume
9
Number
4
Pages
721-731
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
We have quantified activity-dependent uptake of the fluorescent dye FM1-43 in combination with immunocytochemistry for synaptic vesicle-associated proteins (SVPs) at individual synapses in primary cultures of rat cortical neurons. We show that expression of synaptic proteins is highly variable and that the levels of synaptophysin (p38), synapsin I and sv2, but not synapsin II, correlate with the extent of FM1-43 labelling at synapses. The data indicate that SVP levels affect the uptake of FM1-43 with different efficacy (p38 > synapsin I > sv2 or synapsin II). We also found that the relative levels of SVPs vary at individual boutons of single neurons grown in isolation, which indicates that differential regulation of specific SVPs may contribute to the selective modulation of activity at synapses of the same neuron.
Keywords
Animals, Animals, Newborn, Cells, Cultured, Cerebral Cortex/cytology, Cerebral Cortex/physiology, Fluorescent Dyes, Nerve Tissue Proteins/analysis, Nerve Tissue Proteins/biosynthesis, Neurons/physiology, Neurons/ultrastructure, Pyridinium Compounds/pharmacokinetics, Quaternary Ammonium Compounds/pharmacokinetics, Rats, Synapses/physiology, Synapses/ultrastructure, Synapsins/biosynthesis, Synaptic Vesicles/physiology, Synaptic Vesicles/ultrastructure, Synaptophysin/biosynthesis
Pubmed
Web of science
Create date
19/11/2007 12:33
Last modification date
20/08/2019 13:20
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