Host and invader impact of transfer of the clc genomic island into Pseudomonas aeruginosa PAO1

Details

Serval ID
serval:BIB_341690BB4E4B
Type
Article: article from journal or magazin.
Collection
Publications
Title
Host and invader impact of transfer of the clc genomic island into Pseudomonas aeruginosa PAO1
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Gaillard M., Pernet N., Vogne C., Hagenbuchle O., van der Meer J. R.
ISSN
1091-6490
Publication state
Published
Issued date
2008
Peer-reviewed
Oui
Volume
105
Number
19
Pages
7058-7063
Language
english
Abstract
Genomic islands, large potentially mobile regions of bacterial chromosomes, are a major contributor to bacteria evolution. Here, we investigated the fitness cost and phenotypic differences between the bacterium Pseudomonas aeruginosa PAO1 and a derivative carrying one integrated copy of the clc element, a 103-kb genomic island [and integrative and conjugative element (ICE)] originating in Pseudomonas sp. strain B13 and a close relative of genomic islands found in clinical and environmental isolates of P. aeruginosa. By using a combination of whole genome transcriptome profiling, phenotypic arrays, competition experiments, and biofilm formation studies, only few differences became apparent, such as reduced biofilm growth and fourfold stationary phase repression of genes involved in acetoin metabolism in PAO1 containing the clc element. In contrast, PAO1 carrying the clc element acquired the capacity to grow on 3-chlorobenzoate and 2-aminophenol as sole carbon and energy substrates. No fitness loss >1% was detectable in competition experiments between PAO1 and PAO1 carrying the clc element. The genes from the clc element were not silent in PAO1, and excision was observed, although transfer of clc from PAO1 to other recipient bacteria was reduced by two orders of magnitude. Our results indicate that newly acquired mobile DNA not necessarily invoke an important fitness cost on their host. Absence of immediate detriment to the host may have contributed to the wide distribution of genomic islands like clc in bacterial genomes
Keywords
Bacteria , Biofilms , Dna , drug effects , Gene Expression Regulation,Bacterial , genetics , Genome,Bacterial , Genomic Islands , Host-Pathogen Interactions , metabolism , microbiology , Mutagenesis,Insertional , Oligonucleotide Array Sequence Analysis , pharmacology , Phenotype , physiology , Pseudomonas aeruginosa , Rifampin , Rna , RNA,Transfer,Gly , Substrate Specificity , Succinic Acid , Switzerland
Pubmed
Web of science
Open Access
Yes
Create date
29/01/2009 23:13
Last modification date
08/05/2019 16:52
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