Histomorphological Phenotyping of the Adult Mouse Brain.
Details
Serval ID
serval:BIB_3410E4929B2B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Histomorphological Phenotyping of the Adult Mouse Brain.
Journal
Current protocols in mouse biology
ISSN
2161-2617 (Electronic)
ISSN-L
2161-2617
Publication state
Published
Issued date
01/09/2016
Peer-reviewed
Oui
Volume
6
Number
3
Pages
307-332
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Abstract
This article describes a series of standard operating procedures for morphological phenotyping of the mouse brain using basic histology. Many histological studies of the mouse brain use qualitative approaches based on what the human eye can detect. Consequently, some phenotypic information may be missed. Here we describe a quantitative approach for the assessment of brain morphology that is simple and robust. A total of 78 measurements are made throughout the brain at specific and well-defined regions, including the cortex, the hippocampus, and the cerebellum. Experimental design and timeline considerations, including strain background effects, the importance of sectioning quality, measurement variability, and efforts to correct human errors are discussed. © 2016 by John Wiley & Sons, Inc.
Keywords
Animals, Brain/anatomy & histology, Histological Techniques/methods, Mice/anatomy & histology
Pubmed
Create date
16/09/2016 21:45
Last modification date
20/08/2019 14:20