Localization of matrix metalloproteinase 9 to the cell surface provides a mechanism for CD44-mediated tumor invasion.

Details

Serval ID
serval:BIB_33DBE35AD322
Type
Article: article from journal or magazin.
Collection
Publications
Title
Localization of matrix metalloproteinase 9 to the cell surface provides a mechanism for CD44-mediated tumor invasion.
Journal
Genes and Development
Author(s)
Yu Q., Stamenkovic I.
ISSN
0890-9369[print], 0890-9369[linking]
Publication state
Published
Issued date
1999
Volume
13
Number
1
Pages
35-48
Language
english
Abstract
The cell surface hyaluronan receptor CD44 promotes tumor growth and metastasis by mechanisms that remain poorly understood. We show here that CD44 associates with a proteolytic form of the matrix metalloproteinase-9 (MMP-9) on the surface of mouse mammary carcinoma and human melanoma cells. CD44-associated cell surface MMP-9 promotes cell-mediated collagen IV degradation in vitro and mediates tumor cell invasion of G8 myoblast monolayers. Several distinct CD44 isoforms coprecipitate with MMP-9 and CD44/MMP-9 coclustering is observed to be dependent on the ability of CD44 to form hyaluronan-induced aggregates. Disruption of CD44/MMP-9 cluster formation, by overexpression of soluble or truncated cell surface CD44, is shown to inhibit tumor invasiveness in vivo. Our observations indicate that CD44 serves to anchor MMP-9 on the cell surface and define a mechanism for CD44-mediated tumor invasion.
Keywords
Animals, Antigens, CD44/immunology, Collagen/metabolism, Collagenases/metabolism, Endocytosis/physiology, Gene Expression Regulation, Enzymologic/genetics, Gene Expression Regulation, Neoplastic/genetics, Humans, Hyaluronic Acid/metabolism, Immunohistochemistry, Mammary Neoplasms, Experimental/enzymology, Mammary Neoplasms, Experimental/pathology, Matrix Metalloproteinase 9, Melanoma/enzymology, Mice, Neoplasm Invasiveness/physiopathology, Protein Binding, RNA, Messenger/genetics, Transfection/genetics, Tumor Cells, Cultured
Pubmed
Create date
26/08/2010 16:42
Last modification date
20/08/2019 13:20
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