Allogeneic mixed lymphocyte reactions in humans: pretreatment of either the stimulator or the responder cell population with monoclonal anti-Ia antibodies leads to an inhibition of cell proliferation
Details
Serval ID
serval:BIB_33CCA10DDCE5
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Allogeneic mixed lymphocyte reactions in humans: pretreatment of either the stimulator or the responder cell population with monoclonal anti-Ia antibodies leads to an inhibition of cell proliferation
Journal
Journal of Immunology
ISSN
0022-1767 (Print)
Publication state
Published
Issued date
12/1981
Volume
127
Number
6
Pages
2438-42
Notes
Journal Article --- Old month value: Dec
Abstract
We analyzed the effect of 2 hybridoma monoclonal antibodies (Mab), D1-12 and D4-22, with specificity for common determinants of human Ia molecules, on the mixed lymphocyte culture (MLC) response. The results show that addition of either of the 2 Mab as late as day 3 after the onset of the culture completely inhibits the proliferative response generated in MLC. Because the antigenic determinants recognized by the 2 Mab that were used in this study have been shown to belong to distinct Ia molecules, it appears the inhibitory effect observed in MLC containing such Mabs cannot be explained simply by the masking of Ia molecules on the stimulator cell population. In agreement with previous studies by other investigators, treatment of a leukocyte population with the cytolytic D1-12 Mab plus complement strongly reduced its ability to stimulate in MLC. More importantly such a treatment also decreased the ability of a leukocyte population to respond in MLC. In the latter case, the inhibitory effect appears to be directed against T cells since highly purified E-rosetting cells treated with D1-12 plus complement were unable to respond in MLC. The possible implications of these results are discussed.
Keywords
Animals
*Antibodies, Monoclonal
Cell Division
Complement System Proteins
Dose-Response Relationship, Immunologic
Epitopes
Histocompatibility Antigens Class II/*immunology
Humans
Kinetics
Lymphocyte Culture Test, Mixed
Mice
Mice, Inbred BALB C
Rosette Formation
T-Lymphocytes/immunology
Pubmed
Web of science
Create date
28/01/2008 11:13
Last modification date
20/08/2019 13:20