Long-term prognostic significance of HER-2/neu in untreated node-negative breast cancer depends on the method of testing

Details

Serval ID
serval:BIB_33BC121D0AE8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Long-term prognostic significance of HER-2/neu in untreated node-negative breast cancer depends on the method of testing
Journal
Breast Cancer Research
Author(s)
Schmidt  M., Lewark  B., Kohlschmidt  N., Glawatz  C., Steiner  E., Tanner  B., Pilch  H., Weikel  W., Kolbl  H., Lehr  H. A.
ISSN
1465-542X (Electronic)
Publication state
Published
Issued date
2005
Volume
7
Number
2
Pages
R256-R266
Notes
PT - Comparative Study PT - Journal Article
Abstract
INTRODUCTION: The prognostic significance of HER-2/neu in breast cancer is a matter of controversy. We have performed a study in 101 node-negative breast cancer patients with long-term follow-up not treated in the adjuvant setting, and analysed the prognostic significance of immunohistochemistry (IHC) and fluorescence in situ hybridisation (FISH), both separately and in combination, in comparison with traditional prognostic factors. METHODS: Overexpression was classified semiquantitatively according to a score (0 to 3+) (HER-2_SCO). FISH was used to analyse HER2/neu amplification (HER-2_AMP). Patients classified 2+ by IHC were examined with FISH for amplification (HER-2_ALG). Patients with 3+ overexpression as well as amplification of HER-2/neu were positive for the combined variable HER2_COM. These variables were compared with tumour size, histological grade and hormone receptor status. RESULTS: HER-2_SCO was 3+ in 20% of all tumours. HER-2_ALG was positive in 22% and amplification (HER-2_AMP) was found in 17% of all tumours. Eleven percent of the tumours showed simultaneous 3+ overexpression and amplification. Only histological grade (relative risk [RR] 3.22, 95% confidence interval [CI] 1.73-5.99, P = 0.0002) and HER-2_AMP (RR 2.47, 95% CI 1.12-5.48, P = 0.026) were significant for disease-free survival in multivariate analysis. For overall survival, both histological grade (RR 3.89, 95% CI 1.77-8.55, P = 0.0007) and HER-2_AMP (RR 3.08, 95% CI 1.24-7.66, P = 0.016) retained their independent significance. CONCLUSION: The prognostic significance of HER-2/neu in node-negative breast cancer depends on the method of testing: only the amplification of HER-2/neu is an independent prognostic factor for the long-term prognosis of untreated node-negative breast cancer
Keywords
Adult/Aged/Aged,80 and over/Breast Neoplasms/genetics/Pathology/Disease-Free Survival/Female/Follow-Up Studies/Gene Amplification/Gene Expression Profiling/Humans/Immunohistochemistry/In Situ Hybridization,Fluorescence/Lymphatic Metastasis/Middle Aged/Predictive Value of Tests/Prognosis/Receptor,erbB-2/analysis/biosynthesis/Reproducibility of Results
Pubmed
Web of science
Open Access
Yes
Create date
29/01/2008 19:34
Last modification date
20/08/2019 14:20
Usage data