An unbiased correction factor for cell counts in histological sections.

Details

Serval ID
serval:BIB_33B11B695E7E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
An unbiased correction factor for cell counts in histological sections.
Journal
Journal of Neuroscience Methods
Author(s)
Clarke P.G.H.
ISSN
0165-0270[print], 0165-0270[linking]
Publication state
Published
Issued date
08/1993
Volume
49
Number
1-2
Pages
133-140
Language
english
Notes
Journal Article Research Support, Non-U.S. Gov't --- Old month value: Aug
Abstract
It is common procedure to correct raw counts of cells (or nuclei or other such particles) in histological sections by multiplying them by a 'correction factor', to allow for sectioned particles being counted more than once. However, the derivation of the standard formulae assumes that the particles to be counted are of uniform size. Therefore, these formulae may be biased in practical situations. Here, a more general correction factor (C) not depending on this restrictive assumption is derived: C = sigma i = 1 m T/(T - h(i))/sigma i = 1 m (T - R - S + h(i))/(T - h(i)) where T is section thickness, h(i) is the height of particle i measured perpendicular to the section plane, R and S are the heights, assumed constant, of the upper and lower 'lost caps', and the summation is performed over m (about 20) particles sampled randomly from among those lying wholly within individual sections. It is proposed that h(i) be measured by a differential focusing method; the formula will then be valid for particles of variable shape as well as size.
Keywords
Bias (Epidemiology), Cell Count, Cell Nucleus/ultrastructure, Cell Size, Mathematics
Pubmed
Web of science
Create date
20/01/2008 18:49
Last modification date
20/08/2019 14:19
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