Immunocytochemical localization of the glucose transporter 2 (GLUT2) in the adult rat brain. II. Electron microscopic study.

Details

Serval ID
serval:BIB_3395A6F51BC3
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Immunocytochemical localization of the glucose transporter 2 (GLUT2) in the adult rat brain. II. Electron microscopic study.
Journal
Journal of Chemical Neuroanatomy
Author(s)
Arluison M., Quignon M., Thorens B., Leloup C., Penicaud L.
ISSN
0891-0618[print], 0891-0618[linking]
Publication state
Published
Issued date
2004
Volume
28
Number
3
Pages
137-146
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Following a former immunohistochemical study in the rat brain [Arluison, M., Quignon, M., Nguyen, P., Thorens, B., Leloup, C., Penicaud, L. Distribution and anatomical localization of the glucose transporter 2 (GLUT2) in the adult rat brain. I. Immunohistochemical study. J. Chem. Neuroanat., in press], we have analyzed the ultrastructural localization of GLUT2 in representative and/or critical areas of the forebrain and hindbrain. In agreement with previous results, we observe few oligodendrocyte and astrocyte cell bodies discretely labeled for GLUT2 in large myelinated fibre bundles and most brain areas examined, whereas the reactive glial processes are more numerous and often localized in the vicinity of nerve terminals and/or dendrites or dendritic spines forming synaptic contacts. Only some of them appear closely bound to unlabeled nerve cell bodies and dendrites. Furthermore, the nerve cell bodies prominently immunostained for GLUT2 are scarce in the brain nuclei examined, whereas the labeled dendrites and dendritic spines are relatively numerous and frequently engaged in synaptic junctions. In conformity with the observation of GLUT2-immunoreactive rings at the periphery of numerous nerve cell bodies in various brain areas (see previous paper), we report here that some neuronal perikarya of the dorsal endopiriform nucleus/perirhinal cortex exhibit some patches of immunostaining just below the plasma membrane. However, the presence of many GLUT2-immunoreactive nerve terminals and/or astrocyte processes, some of them being occasionally attached to nerve cell bodies and dendrites, could also explain the pericellular labeling observed. The results here reported support the idea that GLUT2 may be expressed by some cerebral neurones possibly involved in glucose sensing, as previously discussed. However, it is also possible that this transporter participate in the regulation of neurotransmitter release and, perhaps, in the release of glucose by glial cells.
Keywords
Animals, Astrocytes/metabolism, Astrocytes/ultrastructure, Brain/metabolism, Brain/ultrastructure, Dendritic Spines/ultrastructure, Glucose Transporter Type 2, Immunohistochemistry, Male, Microscopy, Electron, Transmission, Monosaccharide Transport Proteins/ultrastructure, Oligodendroglia/metabolism, Oligodendroglia/ultrastructure, Rats
Pubmed
Web of science
Create date
24/01/2008 14:41
Last modification date
20/08/2019 14:19
Usage data