Endothelin receptor expression in human lungs of newborns with congenital diaphragmatic hernia.
Details
Serval ID
serval:BIB_33842
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Endothelin receptor expression in human lungs of newborns with congenital diaphragmatic hernia.
Journal
Journal of Pathology
ISSN
0022-3417 (Print)
ISSN-L
0022-3417
Publication state
Published
Issued date
2005
Volume
205
Number
1
Pages
112-118
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
Congenital diaphragmatic hernia (CDH) is a major cause of refractory respiratory failure in the neonatal period and is characterized by persistent pulmonary hypertension of the newborn (PPHN) and pulmonary hypoplasia. Endothelin-1 (ET-1) dysregulation may play a significant role in the pathophysiology of PPHN and ET-1 acts through binding to type A (ETA) and type B (ETB) receptors. Therefore, ETA and ETB receptor protein expression was studied using immunohistochemistry in 10 lung specimens obtained from newborns with CDH, and 4 normal lung specimens, in order to explore whether dysregulation of ETA and ETB expression contributes to PPHN. ETA and ETB mRNAs were then quantified using real-time RT-PCR in laser-microdissected pulmonary resistive arteries. In the lungs of newborns with CDH, immunohistochemistry of both ETA and ETB receptors demonstrated over-expression in the thickened media of pulmonary arteries. Using laser microdissection and real-time RT-PCR, higher levels of ETA and ETB mRNA were found in CDH pulmonary arteries than in controls: this increase was more pronounced for ETA mRNA. This study provides the first demonstration of ET-1 receptor dysregulation in association with structural alteration of pulmonary arteries in newborns with CDH and PPHN. This dysregulation preferentially affects the ETA receptor. These results suggest that dysregulation of ET-1 receptors may contribute to PPHN associated with CDH.
Keywords
Body Weight, Female, Gene Expression, Hernia, Diaphragmatic/complications, Hernia, Diaphragmatic/congenital, Humans, Infant, Newborn, Lung/metabolism, Lung/pathology, Male, Microdissection/methods, Organ Size, Persistent Fetal Circulation Syndrome/etiology, Persistent Fetal Circulation Syndrome/metabolism, Pulmonary Artery/metabolism, RNA, Messenger/genetics, Receptor, Endothelin A/genetics, Receptor, Endothelin A/metabolism, Receptor, Endothelin B/genetics, Receptor, Endothelin B/metabolism, Receptors, Endothelin/metabolism, Retrospective Studies, Reverse Transcriptase Polymerase Chain Reaction
Pubmed
Web of science
Create date
19/11/2007 13:33
Last modification date
20/08/2019 14:19