Appearance of the bona fide spiral tubule of ORF virus is dependent on an intact 10-kilodalton viral protein.

Details

Serval ID
serval:BIB_33770
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Appearance of the bona fide spiral tubule of ORF virus is dependent on an intact 10-kilodalton viral protein.
Journal
Journal of Virology
Author(s)
Spehner D., De Carlo S., Drillien R., Weiland F., Mildner K., Hanau D., Rziha H.J.
ISSN
0022-538X
Publication state
Published
Issued date
2004
Volume
78
Number
15
Pages
8085-8093
Language
english
Notes
Publication types: Journal Article
Abstract
Parapoxviruses can be morphologically distinguished from other poxviruses in conventional negative staining electron microscopy (EM) by their ovoid appearance and the spiral tubule surrounding the virion's surface. However, this technique may introduce artifacts. We have examined Orf virus (ORFV; the prototype species of the Parapoxvirus genus) by cryoelectron microscopy (cryo-EM) and cryo-negative staining EM. From these studies we suggest that the shape and unique spiral tubule are authentic features of the parapoxviruses. We also constructed an ORFV mutant deleted of a gene encoding a 10-kDa protein, which is an orthologue of the vaccinia virus (VACV) 14-kDa fusion protein, and investigated its ultrastructure. This mutant virus multiplied slowly in permissive cells and produced infectious but morphologically aberrant particles. Mutant virions lacked the spiral tubule but displayed short disorganized tubules similar to those observed on the surface of VACV. In addition, thin extensions or loop-like structures were appended to the ORFV mutant particles. We suggest that these appended structures arise from a failure of the mutant virus particles to properly seal and that the sealing activity is dependent on the 10-kDa protein.
Keywords
Animals, Cattle, Cercopithecus aethiops, Humans, Microscopy, Electron, Orf virus/genetics, Orf virus/physiology, Vero Cells, Viral Proteins/physiology, Virus Assembly
Pubmed
Web of science
Open Access
Yes
Create date
19/11/2007 12:32
Last modification date
20/08/2019 13:19
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